{"product_id":"biolegend-405725","title":"Biolegend, 405725, Brilliant Violet 421™ anti-mouse IgD Antibody, 50μg","description":"\u003cp\u003eSurface IgD is an important B cell differentiation marker.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3, and spatial biology (IBEX)10,11.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC) Weih D, et al. 2001. J. Immunol. 167:1909. (IHC) Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC) Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC) Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed Park SY, et al. 2013. J. Immunol. 190:1094. PubMed Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Chei S, et al. 2020. Front Nutr. 7:583186. PubMed Marcial-Juárez E, et al. 2023. iScience. 26:106310. PubMed Worth AN, et al. 2022. Cell Rep. 39:110899. PubMed Yeh CH, et al. 2022. Immunity. 55:272. PubMed Pérez‐Mazliah D et al. 2017. EBioMedicine. 24:216-230 . PubMed Suzuki M, et al. 2021. Sci Adv. 7:. PubMed Chen JS, et al. 2022. Sci Immunol. 7:eabl5652. PubMed Vogel AB, et al. 2021. Nature. 592:283. PubMed Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed Verheijen M, et al. 2020. Cell Rep. 108376:33. PubMed Kiyohara H, et al. 2018. Cell Mol Gastroenterol Hepatol. 7:135. PubMed Shin C, et al. 2016. Immunol Lett. 172: 21-28. PubMed Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed Bastow CR, et al. 2022. Front Immunol. 13:873586. PubMed\u003cbr\u003e\nRRID: AB_2562743 (BioLegend Cat. No. 405725)\u003cbr\u003e\nStructure: Ig family\u003cbr\u003e\nDistribution: B cells\u003cbr\u003e\nFunction: B cell differentiation\u003cbr\u003e\nCell Type: B cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nGene ID: 380797\u003cbr\u003e\nUniProt: View information about IgD on UniProt.org\u003cbr\u003e\nClone: 11-26c.2a\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Immunoglobulin D\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860786073769,"sku":"405725","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-405725","provider":"Iright","version":"1.0","type":"link"}