{"product_id":"biolegend-406516","title":"Biolegend, 406516, APC\/Cyanine7 anti-mouse IgM Antibody, 100μg","description":"\u003cp\u003eIgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse Ig cocktail\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with APC\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. Some formats of the RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Tertilt C, et al. 2009. Infect. Immun. 77:3044. (ELISA) PubMed\u003cbr\u003e\nProduct Citations: Tan J, et al. 2021. iScience. 24(8):102835. PubMed Masle-Farquhar E, et al. 2023. Front Immunol. 14:1095257. PubMed Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed Choi JY, et al. 2020. Proc Natl Acad Sci U S A. 117:6042. PubMed González-Hernández A, et al. 2016. Sci Rep. 6:29496. PubMed Meulder F, et al. 2015. Int J Parasit. 45: 637-646. PubMed Pighi C, et al. 2021. Blood Adv. 5:5239. PubMed Weber G, et al. 2014. J Exp Med. 211:1243. PubMed Ray A, et al. 2015. PLoS One. 10: 0137314. PubMed Singh M, et al. 2020. Cell. 180(5):878-894. PubMed Wittner J, et al. 2022. Mucosal Immunol. 15:668. PubMed Scally S, et al. 2017. Nat Commun. . 10.1038\/s41467-017-01924-3. PubMed González-Hernández A, et al. 2017. Int J Parasitol. 10.1016\/j.ijpara.2017.07.002. PubMed Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed Lehrke MJ, et al. 2021. Elife. 10:. PubMed Grit G, et al. 2014. Vet Parasitol. 202:145. PubMed\u003cbr\u003e\nRRID: AB_10690815 (BioLegend Cat. No. 406515) AB_10660305 (BioLegend Cat. No. 406516)\u003cbr\u003e\nStructure: Ig family\u003cbr\u003e\nDistribution: B cells\u003cbr\u003e\nFunction: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis\u003cbr\u003e\nCell Type: B cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nGene ID: 16019\u003cbr\u003e\nUniProt: View information about IgM on UniProt.org\u003cbr\u003e\nClone: RMM-1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Immunoglobulin M\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866201182377,"sku":"406516","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-406516","provider":"Iright","version":"1.0","type":"link"}