{"product_id":"biolegend-406544","title":"Biolegend, 406544, PE\/Cyanine5 anti-mouse IgM Antibody, 100μg","description":"\u003cp\u003eIgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse Ig cocktail\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Cyanine5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. Some formats of the RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Tertilt C, et al. 2009. Infect. Immun. 77:3044. (ELISA) PubMed\u003cbr\u003e\nProduct Citations: Li C, et al. 2023. STAR Protoc. 102578:4. PubMed\u003cbr\u003e\nRRID: AB_2861023 (BioLegend Cat. No. 406543) AB_2876730 (BioLegend Cat. No. 406544)\u003cbr\u003e\nStructure: Ig family\u003cbr\u003e\nDistribution: B cells\u003cbr\u003e\nFunction: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis\u003cbr\u003e\nCell Type: B cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nGene ID: 16019\u003cbr\u003e\nUniProt: View information about IgM on UniProt.org\u003cbr\u003e\nClone: RMM-1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Immunoglobulin M\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866201510057,"sku":"406544","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-406544","provider":"Iright","version":"1.0","type":"link"}