{"product_id":"biolegend-407105","title":"Biolegend, 407105, FITC anti-mouse IgG2a Antibody, 50μg","description":"\u003cp\u003eThe RMG2a-62 monoclonal antibody reacts with immunoglobulin G2a and G2c (IgG2a, IgG2c) and in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG2a-62 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse Ig cocktail\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Gavin AL, et al.2006. Science 314:1936. Podolin PL, et al. 2008. J. Immunol. 180:7989. (IP) PubMed\u003cbr\u003e\nProduct Citations: Liang Q, et al. 2022. iScience. 25:104043. PubMed Fuchs D, et al. 2010. Biochem Biophys Res Commun. 394:1098. PubMed Gradiz R, et al. 2016. Sci Rep. 6:21648. PubMed East J, et al. 2012. J Vis Exp. 70: 4333. PubMed Lin Y, et al. 2011. Transpl Immunol. 25:194. PubMed Souza SP, et al. 2021. PLoS Pathog. 17:e1010081. PubMed Pandya P, et al. 2020. PLoS One. 15:e0233961. PubMed Cronin J, et al. 2016. Mucosal Immunol. 10.1038\/mi.2015.131. PubMed\u003cbr\u003e\nRRID: AB_493294 (BioLegend Cat. No. 407105) AB_493295 (BioLegend Cat. No. 407106)\u003cbr\u003e\nGene ID: 668478\u003cbr\u003e\nUniProt: View information about IgG2a on UniProt.org\u003cbr\u003e\nClone: RMG2a-62\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Immunoglobulin G2a\u003cbr\u003e\nIsotype: Rat IgG\u003cbr\u003e\nQ: IgG2a gene is deleted in some mouse strains, which ones are they?\u003cbr\u003e\nA: IgG2a gene is deleted in C57Bl\/6, C57Bl\/10, SJL, and NOD mice. It is replaced with IgG2c gene instead.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866198495401,"sku":"407105","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-407105","provider":"Iright","version":"1.0","type":"link"}