{"product_id":"biolegend-407112","title":"Biolegend, 407112, PerCP\/Cyanine5.5 anti-mouse IgG2a Antibody, 100μg","description":"\u003cp\u003eThe RMG2a-62 monoclonal antibody reacts with immunoglobulin G2a and G2c (IgG2a, IgG2c) and in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG2a-62 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse Ig cocktail\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Gavin AL, et al.2006. Science 314:1936. Podolin PL, et al. 2008. J. Immunol. 180:7989. (IP) PubMed\u003cbr\u003e\nProduct Citations: Kimura S, et al. 2020. Nat Commun. 0.620833333. PubMed Parra-Sánchez H, et al. 2018. Mol Immunol. 96:1. PubMed Souza SP, et al. 2021. PLoS Pathog. 17:e1010081. PubMed Hayashi Y, et al. 2017. J Biol Chem. 292(3):1122-1141. PubMed\u003cbr\u003e\nRRID: AB_2562007 (BioLegend Cat. No. 407111) AB_2562008 (BioLegend Cat. No. 407112)\u003cbr\u003e\nGene ID: 668478\u003cbr\u003e\nUniProt: View information about IgG2a on UniProt.org\u003cbr\u003e\nClone: RMG2a-62\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Immunoglobulin G2a\u003cbr\u003e\nIsotype: Rat IgG\u003cbr\u003e\nQ: IgG2a gene is deleted in some mouse strains, which ones are they?\u003cbr\u003e\nA: IgG2a gene is deleted in C57Bl\/6, C57Bl\/10, SJL, and NOD mice. It is replaced with IgG2c gene instead.\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866199183529,"sku":"407112","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-407112","provider":"Iright","version":"1.0","type":"link"}