{"product_id":"biolegend-407307","title":"Biolegend, 407307, PE anti-mouse Ig light chain λ Antibody, 50μg","description":"\u003cp\u003eThe RML-42 monoclonal antibody reacts with immunoglobulin light chain lambda in all tested mouse haplotype (Igh-a and b). It does not react with kappa chain. The RML-42 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse Ig cocktail\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ota M, et al. 2010. J. Immunol. 185:4128. PubMed Xu Z, et al. 2012. J. Immunol. 189:4470. PubMed\u003cbr\u003e\nProduct Citations: Pioli KT, et al. 2023. iScience. 26:106223. PubMed Masle-Farquhar E, et al. 2023. Front Immunol. 14:1095257. PubMed Tian M, et al. 2020. Proc Natl Acad Sci U S A. 117:7929. PubMed Pennington L, et al. 2016. Nat Commun. 7:11610. PubMed McGuire A, et al. 2016. Nat Commun. 7:10618. PubMed Zheng Z, et al. 2020. Cell Reports. 31(13):107819. PubMed Noviski M, et al. 2018. Elife. 7:e35074. PubMed Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed Escolano A, et al. 2016. Cell. 166(6):1445-1458.e12. PubMed\u003cbr\u003e\nRRID: AB_1027660 (BioLegend Cat. No. 407307) AB_1027659 (BioLegend Cat. No. 407308)\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nGene ID: 111519\u003cbr\u003e\nUniProt: View information about Ig light chain lambda on UniProt.org\u003cbr\u003e\nClone: RML-42\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Imunoglobulin light chain lambda\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866200756393,"sku":"407307","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-407307","provider":"Iright","version":"1.0","type":"link"}