{"product_id":"biolegend-410501","title":"Biolegend, 410501, Purified anti-Streptavidin Antibody, 500μg","description":"\u003cp\u003eStreptavidin is a 52 kD tetrameric protein obtained from Streptomyces avidinii . It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10 -14 mol\/L. It is used in a wide range of applications including flow cytometry, molecular biology, and bionanotechnology.\u003cbr\u003e\n500μg\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Streptavidin\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: The 3A20.2 purified antibody is useful as a third step reagent to amplify dim or low signals in many applications. This antibody does not cross-react with avidin.\u003cbr\u003e\nProduct Citations: Trittel S, et al. 2019. Sci Rep. 9:16362. PubMed Andrechak JC, et al. 2022. Cancers (Basel). 14:. PubMed Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed Jiang L, et al. 2018. J Exp Med. 215:1337. PubMed Bartsch YC, et al. 2018. Front Immunol. 1.196527778. PubMed Aparicio-Domingo P, et al. 2015. J Exp Med. 101084\/jm20150318. PubMed Telford W, et al. 2017. Cytometry A. 91:314. PubMed Ribechini E, et al. 2017. Blood Adv. 0.699305556. PubMed\u003cbr\u003e\nRRID: AB_2564569 (BioLegend Cat. No. 410501)\u003cbr\u003e\nStructure: 52 kD tetrameric protein.\u003cbr\u003e\nFunction: Streptavidin binds to biotin with high affinity.\u003cbr\u003e\nLigand\/Receptor: Biotin\u003cbr\u003e\nAntigen References: 1. Dundas CM, et al. 2013. Appl. Microbiol. Biotechnol. 97:9343. 2. Zhao X, et al. 2013. J. Anal Methods Chem. 58:1093. 3. Kaplan DL, et al. 1999. Biomol. Eng. 16:135. 4. Wilbur DS, et al. 1999. Biomol. Eng. 16:113. 5. Sano T, et al. 1998. J. Chromatogr. B. Biomed. Sci. Appl. 715:85.\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nUniProt: View information about Streptavidin on UniProt.org\u003cbr\u003e\nClone: 3A20.2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: SAv\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862384955561,"sku":"410501","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-410501","provider":"Iright","version":"1.0","type":"link"}