{"product_id":"biolegend-426311","title":"Biolegend, 426311, Western-Ready™ Protein Sample Loading Buffer (5X), 20ML","description":"\u003cp\u003eWestern-Ready™ Protein Sample Loading Buffer (5X) can be used for the preparation of samples for non-reducing and reducing SDS-PAGE.\u003cbr\u003e\n20ML\u003cbr\u003e\nFormulation: 300 mM Tris-HCl pH 6.8, 10% Sodium dodecyl sulfate, 30% glycerol, 0.02% Bromophenol blue\u003cbr\u003e\nStorage \u0026amp; Handling: Ambient room temperature\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Dilute Western-Ready™ Protein Sample Loading Buffer (5X) to a 1X concentration (1:4 by volume) using appropriate amount of sample and diluting solution (ie: water, lysis buffer, etc). For sample that are to be used for reducing PAGE, a reducing agent such as as β-ME, DTT, or TCEP must be added to buffer prior to mixing and heating sample.\u003cbr\u003e\nApplication Notes: Western-Ready™ Protein Sample Loading Buffer (5X) contains SDS, which may precipitate at cooler temperatures. If preceipitates are observed in the buffer, heat in 37°C water bath for 5 min to bring SDS back into solution.Western-Ready™ Protein Sample Loading Buffer (5X) is compatible with Bis-Tris, Tris-Glycine, and Tris-Acetate gels.Western-Ready™ Protein Sample Loading Buffer (5X) does not include any reducing agents. For reducing SDS-PAGE, we recommend adding a reducing agent such as ß-ME, DTT, TCEP to buffer prior to mixing with sample.This buffer should not be used for native PAGE.This buffer may be used to elute captured immune complexes during immunoprecipitation-based applications.\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nRegulatory Status: RUO\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866448285865,"sku":"426311","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-426311","provider":"Iright","version":"1.0","type":"link"}