{"product_id":"biolegend-431324","title":"Biolegend, 431324, ELISpot MAX™ Deluxe Set Mouse IL-6, 5plates","description":"\u003cp\u003eMouse IL-6 (Interleukin-6) is a multifunctional cytokine known by various names such as B-cell Stimulatory Factor 2 (BSF2) and Hybridoma Growth Factor (HGF). It is produced by a variety of cells including macrophages, T-cells, osteoblasts, endothelial cells, and hepatocytes. IL-6 plays a pivotal role in both pro-inflammatory and anti-inflammatory responses. It is crucial in numerous biological processes such as B-cell maturation, osteoclast formation, nerve cell differentiation, muscle healing, and muscle hypertrophy. Dysregulation in the expression and activity of Mouse IL-6 can lead to many diseases, including chronic inflammation, cancer, and neurodegenerative disorders.  BioLegend’s ELISpot MAX™ Deluxe Set contains pre-optimized essential reagents and additional buffers and solution for Enzyme-Linked Immunosorbent Spot assay. This kit is designed for detection of mouse IL-6 secreting cells. An antibody specific for mouse IL-6 is coated on ethanol-activated 96-well PVDF membrane plate as the capture. Stimulated and\/or unstimulated cells are pipetted directly into the wells and then incubated at 37˚C for a specific period of time. During this period, capture antibody binds to mouse IL-6 from the secreting cells in the vicinity. Then, cells are removed, and a biotinylated anti-mouse IL-6 detection antibody is added, followed by avidin-HRP. Subsequently, TMB membrane substrate is added. The TMB membrane substrate reacts with HRP to form a blue-black precipitate at the sites of mouse IL-6 secreting cells. The result is visible spots, which corresponds to footprints of mouse IL-6 secreting cells, on the membrane surface.\u003cbr\u003e\n5plates\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nApplication: ELISpot\u003cbr\u003e\nCell Type: Endothelial cells, Macrophages, Osteoblasts, T cells\u003cbr\u003e\nBiology Area: Cancer Biomarkers, Immuno-Oncology, Immunology, Innate Immunity, Neurodegeneration, Signal Transduction\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines, Innate Immune Signaling\u003cbr\u003e\nGene ID: 16193\u003cbr\u003e\nUniProt: View information about IL-6 on UniProt.org\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interleukin 6, B-cell Stimulatory Factor 2 (BSF-2), CTL Differentiation Factor (CDF), Hybridoma Growth Factor\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864267509929,"sku":"431324","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-431324","provider":"Iright","version":"1.0","type":"link"}