{"product_id":"biolegend-437708","title":"Biolegend, 437708, LEGEND MAX™ Free Active TGF-β1 ELISA Kit, 5precoatedplates","description":"\u003cp\u003eTransforming growth factor beta 1 (TGF-β1) is a member of the transforming growth factor beta superfamily of cytokines. TGF-β1 precursor contains 390 amino acids with an N-terminal signal peptide of 29 amino acids required for secretion from a cell, a 249 amino acids pro-region (latency associated peptide or LAP), and a 112 amino acids C-terminal region that becomes the active TGF-β1 upon activation.  Both LAP and TGF-β1 exist as homodimers in circulation, but the disulfide linked homodimers of LAP and TGF-β1 remain non-covalently associated, forming the small latent TGF-β1 complex (SLC, 100 kD).  The large latent TGF-β1 Complex (LLC, 235 - 260 kD) contains a third component, the latent TGF-β binding protein (LTBP), which is linked to LAP by a single disulfide bond.  The LTBP does not confer latency, but for efficient secretion of the complex to extracellular sites.  Free active TGF-β1 can be released (activated) by many factors including enzymes and low or high pH.  TGF-β1 is nearly 100% conserved across mammalian species. It has diverse biological functions in multiple cellular processes such as regulating proliferation and differentiation of various cell types. TGF-β1 is also an important immunoregulatory cytokine, which is involved in the maintenance of self-tolerance, Th17 differentiation, and T cell homeostasis, etc.  It is expected that normal serum, plasma, or other biological fluid contains low concentration of free active TGF-β1 and high concentration of Latent TGF-β1. It is the free active form TGF-β1 that binds TGF-β receptor and exerts biological functions. However, it has been difficult to quantify the free active TGF-β1 because of insufficient sensitivities of most assay products currently available on the market. It is necessary to measure both the free active form and total TGF-β1 in biological samples to understand the TGF-β1 functions.\u003cbr\u003e\n5precoatedplates\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat\u003cbr\u003e\nReported Reactivity: Other species\u003cbr\u003e\nApplication: ELISA\u003cbr\u003e\nAdditional Product Notes: View more applications data for this product in our Scientific Poster Library.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Tucker, TA., et al. 2011. Am J. Respir Cell Mol Biol. 46:173. PubMed Andreade BB, et al. 2013. J Infect Dis. 207:1379. PubMed Wen L, et al. 2014. PLoS One. 9:101841. PubMed Kemter AM, et al. 2015. Life Sci. 15:129. PubMed\u003cbr\u003e\nProduct Citations: Xu M, et al. 2018. Am J Transplant. 18:855. PubMed Gautheron F, et al. 2023. Cell Death Discov. 9:117. PubMed Spinelli FM, et al. 2022. IUBMB Life. 74:927. PubMed Öz HH, et al. 2022. Cell Rep. 41:111797. PubMed Kemter A, et al. 2015. Life Sci. 15:129. PubMed Lawson J, et al. 2016. Vet J. 214:1-6. PubMed Takeshima T, et al. 2016. Proc Natl Acad Sci U S A. 113: 11300 - 11305. PubMed Kupani M, et al. 2021. Front Cell Infect Microbiol. 614165:10. PubMed Lefebvre J, et al. 2016. Sci Rep. 6: 25051. PubMed McInerney-Leo A, et al. 2016. J Med Genet. 53: 457 - 464. PubMed Andrade B, et al. 2013. J Infect Dis. 207:1379. PubMed Díaz-García E, et al. 2021. Cancers (Basel). 13:. PubMed Li Y, et al. 2021. Sci Transl Med. 13:. PubMed Smith C, et al. 2015. Cell Rep. 13:1137-48. PubMed Wen L, et al. 2014. PLoS One. 9:101841. PubMed Tucker T, et al. 2012. Am J Respir Cell Mol Biol. 118:2784. PubMed Tang R, et al. 2020. Exp Ther Med. 3.388194444. PubMed Popek M, et al. 2022. Int J Mol Sci. 23:. PubMed\u003cbr\u003e\nSensitivity: 2.3 pg\/mL\u003cbr\u003e\nStandard Range: 7.8-500 pg\/mL\u003cbr\u003e\nCell Sources: Many cell types, highly expressed on activated Tregs and platelets\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Signal Transduction\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines, Growth Factors\u003cbr\u003e\nGene ID: 21803\u003cbr\u003e\nUniProt: View information about TGF-beta1 on UniProt.org\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Free Active TGF-β1 Pre-coated ELISA Kit\u003cbr\u003e\nQ: In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?\u003cbr\u003e\nA: We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.\u003cbr\u003e\nQ: I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?\u003cbr\u003e\nA: The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.\u003cbr\u003e\nQ: For some of your ELISA kits, why do my serum samples require dilution with assay buffer?\u003cbr\u003e\nA: In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864277045417,"sku":"437708","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-437708","provider":"Iright","version":"1.0","type":"link"}