{"product_id":"biolegend-439209","title":"Biolegend, 439209, RAPID MAX™ Human Granzyme B ELISA Kit, 1precoatedplate","description":"\u003cp\u003eHuman Granzymes are serine proteases that are released by cytoplasmic granules within cytotoxic T cells and natural killer (NK) cells. Human Granzyme B is one of the five members of the human granzyme family which includes Granzymes A, B, G, H, and K. Granzyme B is expressed as a 32 kD protein encoded by the GZMB gene. While Granzyme B is mostly expressed by CTL (Cytotoxic T Lymphocyte) and NK cells, it is also expressed in resting and activated plasmacytoid dendritic cells. Granzyme B is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. Granzyme B and perforin have been shown to induce CTL-mediated target cell DNA fragmentation and apoptosis. Once released from the CTL, Granzyme B binds its receptor, the mannose-6-phosphate\/insulin-like growth factor II receptor, and is endocytosed but remains arrested in endocytic vesicles until released by perforin. Once in the cytosol, Granzyme B targets caspase-3 and other caspases directly or indirectly through the mitochondria, initiating the caspase cascade which leads to DNA fragmentation and apoptosis. Elevated Granzyme B levels have been linked with various disease processes.  The BioLegend RAPID MAX™ Human Granzyme B ELISA Kit is a one step, 75-minute enzyme-linked immunosorbent sandwich assay (ELISA) that includes a 96-well strip plate that is pre-coated with an anti-tag antibody. Standards and samples are added into the wells, followed by an antibody cocktail. The antibody cocktail consists of a tag-conjugated capture antibody and an HRP-conjugated detection antibody. This kit is specifically designed for the accurate quantitation of human Granzyme B cell culture supernatant. This kit is analytically validated with ready-to-use reagents\u003cbr\u003e\n1precoatedplate\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nApplication: ELISA\u003cbr\u003e\nSensitivity: 9.5 ± 7.2 pg\/mL\u003cbr\u003e\nStandard Range: 78-5000 pg\/mL\u003cbr\u003e\nCell Type: Dendritic cells, Granulocytes, NK cells, T cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience\u003cbr\u003e\nMolecular Family: Enzymes and Regulators, Proteases\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nUniProt: View information about Granzyme B on UniProt.org\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, CTLA1, Serine Protease B, CSP-B, CSPB, Cytotoxic serine protease B, CCP1, Cytotoxic cell protease 1\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866442911913,"sku":"439209","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-439209","provider":"Iright","version":"1.0","type":"link"}