{"product_id":"biolegend-439407","title":"Biolegend, 439407, LEGEND MAX™ Mouse IFN-β ELISA Kit, 1precoatedplate","description":"\u003cp\u003eInterferon-β is a member of the type I interferon class. Type I IFNs are involved in the innate immune system, being released non-specifically during the early stage of infection in response to stimuli such as viruses, dsRNA, LPS, and bacterial components. Once secreted by virus-infected or activated cells, they bind to the IFNα\/β receptor (IFNAR), which is found on almost all cell types. Then, via the JAK-STAT pathway, they induce the expression of hundreds of IFN-stimulated genes (ISGs). While the function of many of these proteins remains to be elucidated, a hallmark of type I IFNs is their anti-viral activities, both general and specific against particular viruses. Type I IFNs also regulate cell growth and apoptosis, and exert various immunomodulatory and inflammatory effects. As such, they are clinically relevant, both as therapeutics and as targets of inhibition for the treatment of various autoimmune diseases and cancer.   Mouse IFN-β is a ~20 kD monomeric glycoprotein. It has 50% amino acid sequence homology with mouse IFN-α, its closest related protein, and 62% homology with human IFN-β. IFN-β is currently the therapeutic of choice for treating multiple sclerosis.  BioLegend’s LEGEND MAX™ Mouse IFN-β ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a specific antibody is pre-coated on a 96-well strip-well plate.  BioLegend’s LEGEND MAX™ Mouse IFN-β ELISA Kit is specifically designed for the accurate quantitation of mouse IFN-β from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.\u003cbr\u003e\n1precoatedplate\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nApplication: ELISA\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Shah S, et al. 2013. J. Immunol. 191:3514. PubMed Wang S, et al. 2013. J Virol. 87:11851. PubMed\u003cbr\u003e\nProduct Citations: Casazza RL, et al. 2022. MBio. 13:e0385721. PubMed Wang Y, et al. 2022. Cell Rep. 41:111434. PubMed Park JH, et al. 2022. Cell Rep. 41:111594. PubMed Wei K, et al. 2023. Signal Transduct Target Ther. 8:22. PubMed Oldenburg R, et al. 2018. Front Immunol. 9:2. PubMed Xie X, et al. 2021. BMC Med. 19:247. PubMed Zhou C, et al. 2020. Immunity. 52:767. PubMed Shah S, et al. 2013. J Immunol. 191:3514. PubMed Yan S, et al. 2018. J Immunol. 200:1016. PubMed Zhou P, et al. 2018. Nat Commun. 9:1243. PubMed Zhao M, et al. 2021. PLoS Pathog. 17:e1009901. PubMed Klopfenstein N, et al. 2021. PLoS Pathog. 17:e1009387. PubMed Cheng WY, et al. 2018. Front Immunol. 9:1297. PubMed Yoshida A, et al. 2018. J Virol. 92:e02094. PubMed Wu D, et al. 2022. Cell Death Dis. 13:731. PubMed Piersma SJ, et al. 2020. Elife. 9:00. PubMed Jiang H, et al. 2016. J Immunol. 197: 2880 - 2890. PubMed Kwon HK, et al. 2019. Exp Mol Med. 51:50. PubMed Ferri F, et al. 2015. Nat Commun. 6: 8900. PubMed Fritzlar S, et al. 2019. MBio. 10:e00960. PubMed Synn CB, et al. 2022. Yonsei Med J. 63:42. PubMed Stavrou S, et al. 2018. MBio. 9:e00923. PubMed Chen Y, et al. 2021. Front Immunol. 12:672165. PubMed Li K, et al. 2021. Sci Adv. 7: . PubMed Srikanth S, et al. 2019. Nat Immunol. 20:152. PubMed Kim KS, et al. 2021. J Leukoc Biol. 109:865. PubMed Reimer E, et al. 2020. J Cell Sci. 134:00:00. PubMed Nanda S, et al. 2016. J Immunol. 197(11):4266-4273. PubMed Sousa J, et al. 2020. Nat Commun. 11:1949. PubMed Shepardson K, et al. 2016. MBio. 7: e00506-16. PubMed Wu Y, Ren D, Chen G 2016. J Immunol. 197: 3336 - 3347. PubMed Zhu M, et al. 2015. J Immunol. 195: 289 - 297. PubMed Huang F, et al. 2021. Front Immunol. 12:707298. PubMed Raychowdhury R, et al. 2021. Immunohorizons. 5:818. PubMed Qiu W, et al. 2021. Nat Commun. 1582:12. PubMed Khan M, et al. 2020. PLoS Pathog. 16:e1009020. PubMed Chen X, et al. 2021. J Immunol. :. PubMed Lv M, et al. 2015. Sci Rep. 5: 12954. PubMed Wang L, et al. 2022. Nat Commun. 13:7107. PubMed Yang S, et al. 2019. Sci Adv. 5:eaav7999. PubMed Li X, et al. 2022. Sci Rep. 12:17058. PubMed Zhang Y, et al. 2022. Nat Commun. 13:4948. PubMed Saddawi-Konefka R, et al. 2022. Nat Commun. 13:4298. PubMed Karki R, et al. 2020. J Immunol. 204:2514. PubMed Wang S, et al. 2013. J Virol. 87:11851. PubMed Tong Y, et al. 2018. EBioMedicine. 39:132. PubMed\u003cbr\u003e\nSensitivity: 1.9 pg\/mL\u003cbr\u003e\nStandard Range: 7.8-500 pg\/mL\u003cbr\u003e\nCell Sources: Fibroblasts, epithelial cells\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Signal Transduction\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nGene ID: 15977\u003cbr\u003e\nUniProt: View information about IFN-beta on UniProt.org\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Fibroblast interferon (Fi-IFN, F-IFN), Type-1 interferon, acid-stable interferon, R1-GI factor, IFN-β Pre-coated ELISA Kit\u003cbr\u003e\nQ: In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?\u003cbr\u003e\nA: We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.\u003cbr\u003e\nQ: I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?\u003cbr\u003e\nA: The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.\u003cbr\u003e\nQ: For some of your ELISA kits, why do my serum samples require dilution with assay buffer?\u003cbr\u003e\nA: In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864321151145,"sku":"439407","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-439407","provider":"Iright","version":"1.0","type":"link"}