{"product_id":"biolegend-500604","title":"Biolegend, 500604, Biotin anti-human IL-3 Antibody, 500μg","description":"\u003cp\u003eIL-3 is a highly species-specific pleiotropic factor produced primarily by activated T cells though also by mast cells, keratinocytes, and astrocytes, which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. The BVD3-1F9 antibody can neutralize the bioactivity of natural or recombinant IL-3.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Yeast-expressed, recombinant human IL-3\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: ELISA Detection, ELISPOT Detection\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.5-2.0 µg\/ml is recommended. To obtain a linear standard curve, serial dilutions of human IL-3 protein ranging from 2000 to 15 pg\/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: ELISA1-3,6 or ELISPOT4 Detection: The biotinylated BVD3-1F9 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified BVD8-3G11 (Cat. No. 500502) antibody as the capture antibody.Flow Cytometry: The fluorochrome-labelled BVD3-1F9 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-3 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.Additional reported applications (for the relevant formats) include: immunoprecipitation6, Western blotting6, neutralization1,6, immunohistochemical staining5,7 of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Abrams J, et al. 1992. Immunological Reviews 127:5. Abrams J, et al. 1994. Eosinophils in Allergy and Inflammation. Marcel Dekker New York. p.133. Abrams J. 1995. Curr. Prot. Immunol.. 6.20. Mahanty S, et al. 1992. J. Immunol. 148:3567. Andersson U, et al. 1993. Detection and quantification of gene expression. New York: Springer-Verlag. Kaushansky K. 1992. J. Clin. Invest. 90:1879. Andersson J, et al. 1994. Immunology 83:16.\u003cbr\u003e\nRRID: AB_2123713 (BioLegend Cat. No. 500604)\u003cbr\u003e\nStructure: Cytokine; 14-30 kD (Mammalian)\u003cbr\u003e\nBioactivity: Proliferation\/differentiation of almost all types of hematopoietic progenitors into granulocytes, macrophages, erythroid cells, megakaryocytes, mast cell colonies; induces expression of 20-α-steroid dehydrogenase, histidine, ornithine decarboxylase\u003cbr\u003e\nCell Sources: Activated T cells, mast cells, eosinophils, keratinocytes, NK cells, endothelial cells\u003cbr\u003e\nCell Targets: Erythroid cells, megakaryocytes, neutrophils, eosinophils, basophils, mast cells, monocytic lineages\u003cbr\u003e\nReceptors: IL-3R, α subunit (CD123), common β subunit (CDw131)\u003cbr\u003e\nBiology Area: Cell Biology, Stem Cells\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines, Growth Factors\u003cbr\u003e\nAntigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Frendl G. 1992. Int. J. Immunopharmacol. 14:421. 3. Ihle J. 1992. Chem. Immunology 51:65.\u003cbr\u003e\nRegulation: Upregulated by T cell stimulation; downregulated by glucocorticoids\u003cbr\u003e\nGene ID: 3562\u003cbr\u003e\nUniProt: View information about IL-3 on UniProt.org\u003cbr\u003e\nClone: BVD3-1F9\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interleukin-3, Burst promoting activity, Eosinophil colony stimulating factor (Eo-CSF), Hematopoietic cell growth factor (HCGF), Mast (MGF\/MCGF), Multi-colony stimulating (Multi-CSF), P cell stimulating activity (PCSA), Thy1 inducing factor\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862930903209,"sku":"500604","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-500604","provider":"Iright","version":"1.0","type":"link"}