{"product_id":"biolegend-501811","title":"Biolegend, 501811, APC anti-human IL-12\/IL-23 p40 (monomer, dimer, heterodimer) Antibody, 50μg","description":"\u003cp\u003eThe C11.5 antibody reacts with human IL-12 p40 subunit of the IL-12 p70 heterodimer and IL-23 p40 subunit of the IL-23 p19\/p40, as well as p40 monomer and homodimer. The C11.5 antibody can neutralize the bioactivity of natural or recombinant IL-12 p70.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: CHO-expressed, recombinant human IL-12 p70 heterod\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Flow Cytometry3,5: The fluorochrome-labeled C11.5 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-12 p40 (monomeric, heterodimeric, homodimeric) -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section. Neutralization6: The LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 501813). Additional reported applications (for the relevant formats) include: Western blotting, ELISA Capture1,2, ICFC Blocking, immunohistochemical staining4 of paraformaldehyde-fixed, saponin-treated tissue frozen sections, and immunocytochemistry. Note: For testing human IL-12\/IL-23 p40 (monomer, dimer) in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430701 to 430706) are specially developed and recommended. For testing human IL-12 p70 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 431701 to 431706) are specially developed and recommended.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): D'Andrea A, et al. 1992. J. Exp. Med. 176:1387. D'Andrea A, et al. 1993. J. Exp. Med. 178:1041. Prussin C, et al. 1995. J. Immunol. Meth. 188:117. Leifeld L, et al. 2002. Hepatology 36:1001. Pekkari K, et al. 2001. Blood 97:3184. Bolovan-Fritts CA, et al. 2004. J. Virol. 78:13173.\u003cbr\u003e\nProduct Citations: Rice TF, et al. 2021. EBioMedicine. 72:103612. PubMed Alameddine J, et al. 2019. Front Immunol. 0.515972222. PubMed Krogmann A, et al. 2016. PLoS One. 11: 0146326. PubMed\u003cbr\u003e\nRRID: AB_315191 (BioLegend Cat. No. 501809)\u003cbr\u003e\nStructure: Cytokine; monomer, heterodimer (p40:p35 or p40:p19) or homodimer (p40:p40)\u003cbr\u003e\nBioactivity: IL-12 p70 (p40:p35) induces IFN-γ, TNF-a production in T and NK cells; costimulation of PBL proliferation; proliferation\/differentiation of TH1 T lymphocytes. IL-23 (p40:p19) induces proliferation and production of IFN-γ by human memory T cells, but not n\u003cbr\u003e\nCell Sources: Dendritic cells, monocytes\/macrophages, B cells, T cells\u003cbr\u003e\nCell Targets: T cells, NK cells, PBL\u003cbr\u003e\nReceptors: IL-12Rß1 binds p40; dimeric with IL-12Rß2 binds p35\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Quesniaux V. 1992. Research Immunol. 143:385. 3. Trinchieri G, et al. 1992 Prog. Growth Factor Res. 4:355. 4. Trinchieri G, et al. 1993 Immunol. Today. 14:335. 5. Oppmann B, et al. 2000 Immunity. 13:715. 6. Aggarwal S, et al. 2003 J. Biol. Chem. 278:1910. 7. Parham C, et al. 2002 J. Immunol. 168:5699. 8. Belladonna ML, et al. 2002 J. Immunol. 168:5448. 9. Lankford CS, et al. 2003 J. Leukoc. Biol. 73:49.\u003cbr\u003e\nRegulation: Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function; p40 subunit in common with IL-23\u003cbr\u003e\nGene ID: 3593\u003cbr\u003e\nUniProt: View information about IL-12\/IL-23 p40 on UniProt.org\u003cbr\u003e\nClone: C11.5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interleukin-12 p40, Interleukin-23 p40, Cytotoxic lymphocyte maturation factor (CLMF), Natural killer cell stimulatory factor (NKSF), CTL maturation factor (TcMF), T-cell stimulating factor (TSF)\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862938800297,"sku":"501811","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-501811","provider":"Iright","version":"1.0","type":"link"}