{"product_id":"biolegend-503104","title":"Biolegend, 503104, Biotin anti-human LT-α (TNF-β) Antibody, 500μg","description":"\u003cp\u003eLymphotoxin-α (LT-α), also known as tumor necrosis factor-beta (TNF-β), is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. LT-α possesses a signal peptide sequence and is a secreted protein; however, LT-α is also present on the surface of activated T, B and LAK cells as a complex with LT-β. Bioactive LT-α exists as a homotrimer.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: E. coli expressed, recombinant human LT-α.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: ELISA Detection - Quality testedELISPOT Detection, ICFC - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.25-1.0 µg\/ml is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: ELISA or ELISPOT Detection1,2: The biotinylated 359-81-11 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 359-238-8 antibody (Cat. No. 503002\/503004) as the capture antibody.Flow Cytometry3: The fluorochrome-labeled 359-81-11 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify LT-α -producing cells within mixed cell populations. View intracellular cytokine staining protocol.Neutralization1,2: The 359-81-11 antibody can neutralize the bioactivity of natural or recombinant LT-α. The LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human LT-α bioactivity (Cat. No. 503108).Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Meager A, et al. 1987. J. Immunol. Methods 104:31. Meager A, et al. 1987. Hybridoma. 6:305. Jason J, et al. 1999. Clin. Diagn. Lab Immunol. 6:73.\u003cbr\u003e\nRRID: AB_315274 (BioLegend Cat. No. 503104)\u003cbr\u003e\nStructure: TNF superfamily; trimer; 25 kD (Mammalian).\u003cbr\u003e\nFunction: Transformed cell cytotoxicity; mediator of inflammatory and immune functions; fibroblast synthesis of GM-CSF, G-CSF, IL-1, collagenase, prostaglandin E2; monocyte terminal differentiation, synthesis of G-CSF; neutrophil chemoattractant, production of reactive oxygen, fibroblast proliferation.\u003cbr\u003e\nCell Sources: Activated T and B cells, fibroblasts, astrocytes, myeloma, endothelial cells, epithelial cells.\u003cbr\u003e\nCell Targets: Monocytes, B cells, fibroblasts, neutrophils, osteoclasts, keratinocytes, endothelial cells.\u003cbr\u003e\nReceptors: TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75).\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Aggarwal, B., et al.Eds. 1992. Tumor necrosis factors:structure, function, and mechanism of action. Marcel Dekker Inc. 3. Bonavida, B., et al.Eds. 1990. Tumor necrosis factor:structure, mechanisms of action, role in disease and therapy. Karger, Basel. 4. Paul, N., et al. 1987. Annu. Rev. Immunol. 6:407.\u003cbr\u003e\nRegulation: Type II integral membrane protein, forms heterotrimer with type II integral membrane protein LT-β either as LTα1β2 or LTα2β1; processed secreted form is trimeric.\u003cbr\u003e\nGene ID: 4049\u003cbr\u003e\nUniProt: View information about LT-alpha on UniProt.org\u003cbr\u003e\nClone: 359-81-11\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Lymphotoxin-α (LT- α), Tumor necrosis factor-β (TNF-β), Coley's toxin, Hemorrhagic factor, Necrosin, Natural killer cytotoxic factor (NKCF), Differentiation inducing factor (DIF), TNFSF-1\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862379614377,"sku":"503104","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-503104","provider":"Iright","version":"1.0","type":"link"}