{"product_id":"biolegend-506604","title":"Biolegend, 506604, Purified anti-human IL-7 Antibody, 500μg","description":"\u003cp\u003eIL-7 is a potent lymphoid cell growth factor which promotes the proliferation of pre-B cells, thymocytes, T cell progenitors, and mature T cells. IL-7 induces formation of LAK cells and CTLs, and can stimulate tumoricidal activity of monocytes and macrophages. IL-7 can induce upregulation of proinflammatory cytokines in myeloid cells. IL-7 is expressed by stromal cells. The BVD10-11C10 monoclonal antibody is specific for human interleukin-7 (IL-7).\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: E. coli - expressed, recombinant human IL-7\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: ELISA Detection, ELISPOT Detection\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 1-4 µg\/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-7 recombinant protein ranging from 500 to 4 pg\/ml are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 2-8 µg\/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: ELISA or ELISPOT Detection: The biotinylated BVD10-11C10 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified BVD10-40F6 antibody (Cat. No. 501302\/501304) as the capture antibody.Additional reported applications (for the relevant formats) include: The BVD10-11C10 is a non-neutralizing antibody.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Abrams J, et al. 1992. Immunol. Rev. 127:5. Abrams J, et al. 1995. Curr. Prot. Immunol. John Wiley and Sons New York.\u003cbr\u003e\nRRID: AB_2125818 (BioLegend Cat. No. 506604)\u003cbr\u003e\nStructure: Cytokine; 20-28 kD (Mammalian)\u003cbr\u003e\nBioactivity: Proliferation of pre-\/pro-B cells, thymocytes, T cells; formation of LAK cells, CTLs, stimulation of tumoricidal activity monocytes\/macrophages; upregulate proinflammatory cytokines in myeloid cells\u003cbr\u003e\nCell Sources: Adherent bone marrow stromal cells, thymic stromal cells, spleen, keratinocytes\u003cbr\u003e\nReceptors: Heterodimer IL-7Rα (CD127); γ-subunit (CD132) in common with IL-2R, IL-4R, IL-13R, IL-15R\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Park L, et al. 1992. Adv. Exp. Med. Biol. 323 125. 3. Melchers F, et al. 1992. Adv. Exp. Med. Biol. 323 111.\u003cbr\u003e\nRegulation: Downregulated by TGF-β\u003cbr\u003e\nGene ID: 3574\u003cbr\u003e\nUniProt: View information about IL-7 on UniProt.org\u003cbr\u003e\nClone: BVD10-11C10\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interleukin-7, Lymphopoietin-1 (LP-1), Pre-B cell growth factor, Thymocyte growth factor\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862929297577,"sku":"506604","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-506604","provider":"Iright","version":"1.0","type":"link"}