{"product_id":"biolegend-508301","title":"Biolegend, 508301, Biotin anti-human IL-1β Antibody, 50μg","description":"\u003cp\u003eIL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1β is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. The JK1B-2 antibody reacts with human interleukin-1β (IL-1β) and baboon IL-1β. The JK1B-2 antibody does not recognize mouse IL-1β or human IL-1α or other cytokines. The JK1B-2 antibody can neutralize the bioactivity of natural or recombinant IL-1β.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant human IL-1 beta\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: ELISA Detection, ELISPOT Detection\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 1-4 µg\/ml is recommended. To obtain a linear standard curve, serial dilutions of human IL-1β protein ranging from 250 to 2 pg\/ml are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 2-8 µg\/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: ELISA or ELISPOT Detection: The biotinylated JK1B-2 antibody is useful as the detection antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified JK1B-1 antibody (Cat. No. 508202\/508204) as the capture antibody.\u003cbr\u003e\nProduct Citations: Verigos J, et al. 2019. Cancers (Basel). :11. PubMed Ghait M, et al. 2022. Eur J Immunol. 52:907. PubMed Kamperman T et al. 2019. Nat Commun. 10(1):4347 . PubMed\u003cbr\u003e\nRRID: AB_315512 (BioLegend Cat. No. 508301) AB_315513 (BioLegend Cat. No. 508302)\u003cbr\u003e\nStructure: Cytokine; 17.3 kD (Mammalian)\u003cbr\u003e\nCell Sources: Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma, keratinocytes,\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nGene ID: 3553\u003cbr\u003e\nUniProt: View information about IL-1beta on UniProt.org\u003cbr\u003e\nClone: JK1B-2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interleukin-1β, Catabolin, Hematopoietin-1 (H1), IFNβ-inducing factor, Interleukin-β, Osteoclast activating factor (OAF)\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866458673321,"sku":"508301","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-508301","provider":"Iright","version":"1.0","type":"link"}