{"product_id":"biolegend-509701","title":"Biolegend, 509701, Purified anti-mouse\/human NGF Antibody, 50μg","description":"\u003cp\u003eNerve growth factor (NGF) is a small secreted protein that is important for the growth, maintenance, and survival of certain target neurons (nerve cells). NGF consists of three types of subunits, α, β and γ, which specifically interact to form a 7S, 130,000 molecular weight complex. This complex contains two identical 118-amino acid β-chains, which are solely responsible for nerve growth stimulating activity of NGF. NGF is essential for the development and phenotypic maintenance of neurons in the peripheral nervous system (PNS) and for the functional integrity of cholinergic neurons in the central nervous system (CNS).\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse, Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: recombinant human NGF (a.a. 46-62)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: ELISA Capture - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA applications, a concentration range of 6 - 10 µg\/ml is recommended. Additionally, BioLegend recommends the usage of Tris-Buffered Saline (TBS), pH = 7.2 for all washes and incubations, except substrate buffer. Do not use PBS as results may vary dramatically. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: ELISA Capture: The purified JKmNGF-1 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated JKhNGF-2 antibody (Cat. No. 509802) as the detecting antibody for detecting mouse NGF.\u003cbr\u003e\nRRID: AB_315543 (BioLegend Cat. No. 509701) AB_2282783 (BioLegend Cat. No. 509702)\u003cbr\u003e\nStructure: Kallikrein family of serine proteases; dimer; 13.5 kD (Mammalian)\u003cbr\u003e\nBioactivity: Activates JNK and NF-κB; involved in bone resorption; induces the expression of IL-1, IL-6, IL-12, IL-15 in dendritic cells\u003cbr\u003e\nCell Sources: Hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells, eosinophils\u003cbr\u003e\nCell Targets: Neurons, B cells, mast cells, eosinophils\u003cbr\u003e\nReceptors: Low-affinity NGF receptor (LNGFR, p75 LNGFR, fast NGF receptor); high-affinity NGF receptor (trkA)\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines, Growth Factors\u003cbr\u003e\nAntigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Levi A, et al. 1991. Annu. Rev. Pharmacol. Toxicol. 31:205. 3. Varon S, et al. 1991. Adv. Exp. Med. Biol. 296:267. 4. Micera A, et al. 2003. Cytokine Growth Factor Rev. 14:369.\u003cbr\u003e\nRegulation: Upregulated by glutamate, vitamin D3, IL-6, FGF-basic, astrocyte-specific IL-1, TNF-α, PDGF, TGF-β; downregulated by GABAergic neuronal activity, glucocorticoids, Schwann cell-specific TGF-β\u003cbr\u003e\nGene ID: 180494803\u003cbr\u003e\nUniProt: View information about NGF on UniProt.org\u003cbr\u003e\nClone: JKmNGF-1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Nerve growth factor, NGF, β-NGF\u003cbr\u003e\nIsotype: Mouse IgG1\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866459033769,"sku":"509701","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-509701","provider":"Iright","version":"1.0","type":"link"}