{"product_id":"biolegend-510801","title":"Biolegend, 510801, Purified anti-mouse TNF-α Antibody, 50μg","description":"\u003cp\u003eTNF-α is secreted by macrophages, monocytes, neutrophils, T-cells, and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric mouse TNF-α is 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD protein. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro , hemorraghic necrosis of tumors in vivo , increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. The 6B8 antibody reacts with mouse and rat tumor necrosis factor-α (TNF-α).\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: recombinant mouse TNF-α\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: ELISA Capture - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2-6 µg\/ml is recommended. To obtain a linear standard curve, serial dilutions of mouse TNF-α recombinant protein ranging from 500 to 4 pg\/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: ELISA or ELISPOT Capture: The purified 6B8 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP6-XT22 antibody (Cat. No. 506312) as the detecting antibody. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture. Note: For testing mouse TNF-a in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430901 to 430906) are specially developed and recommended.\u003cbr\u003e\nProduct Citations: Hao J, et al. 2022. Cell Rep. 41:111804. PubMed Lee J, et al. 2015. PLoS One. 10: 0142624. PubMed Oyler‐Yaniv J et al. 2017. Molecular cell. 66(5):635-647 . PubMed Ranjan K, et al. 2021. J Clin Invest. 131:. PubMed Jain A, et al. 2020. Nat Immunol. 0.920138889. PubMed\u003cbr\u003e\nRRID: AB_315566 (BioLegend Cat. No. 510801) AB_315567 (BioLegend Cat. No. 510802)\u003cbr\u003e\nStructure: TNF superfamily; dimer\/trimer; 18-150 kD (Mammalian)\u003cbr\u003e\nBioactivity: Paracrine\/endocrine mediator of inflammatory and immune functions; selectively cytotoxic for transformed cells; endothelial cell alterations; chemoattractant\u003cbr\u003e\nCell Sources: Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells\u003cbr\u003e\nCell Targets: Monocytes, neutrophils, macrophages, T cells, fibroblasts, endothelial cells, osteoclasts, adipocytes, astroglia, microglia\u003cbr\u003e\nReceptors: TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75)\u003cbr\u003e\nCell Type: Tregs\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Beutler B, et al. 1988. Annu. Rev. Biochem. 57:505. 3. Beutler B, et al. 1989. Annu. Rev. Immunol. 7:625. 4. Tracey K, et al. 1993. Crit. Care Med. 21:S415.\u003cbr\u003e\nRegulation: Type II integral membrane protein processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, PAF antagonists,\u003cbr\u003e\nGene ID: 21926\u003cbr\u003e\nUniProt: View information about TNF-alpha on UniProt.org\u003cbr\u003e\nClone: 6B8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF-2, TNF-a, TNF-alpha\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862473593001,"sku":"510801","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-510801","provider":"Iright","version":"1.0","type":"link"}