{"product_id":"biolegend-511401","title":"Biolegend, 511401, Purified anti-human IL-8 Antibody, 50μg","description":"\u003cp\u003eIL-8, also known as neutrophil chemotactic factor, neutrophil activating protein, and monocyte-derived neutrophil chemotactic factor, is a member of the alpha (C-X-C) subfamily of chemokines called CXCL8. In response to proinflammatory stimuli, IL-8 is produced by monocytes, macrophages, T cells, neutrophils, and fibroblasts. IL-8 promotes neutrophil chemotaxis and degranulation. The 72 amino acid IL-8 is the predominant form secreted by monocytes and lymphocytes. The E8N1 antibody recognizes the human IL-8 protein and has been shown to be useful for intracellular immunofluorescence flow cytometric analysis and as ELISA detection antibody.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: ELISA\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.25-1.0 µg\/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-8 recombinant protein ranging from 2000 to 15 pg\/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: ELISA Detection: The biotinylated E8N1 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified H8A5 (Cat. No. 511502) antibody as the capture antibody. Note: For testing human IL-8 in serum or plasma, BioLegend's ELISA Max™ Sets are specially developed and recommended.\u003cbr\u003e\nProduct Citations: Gogalic S, et al. 2018. Biosensors (Basel). 9:1593. PubMed Diray-Arce J, et al. 2023. Cell Rep Med. 4:101079. PubMed Yao Y, et al. 2017. Cytometry B Clin Cytom. 92:12:00. PubMed Salerno–Goncalves R, et al. 2019. PLoS Negl Trop Dis. 13:e0007650. PubMed Stensland ZC, et al. 2022. iScience. 25:103626. PubMed Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed\u003cbr\u003e\nRRID: AB_893460 (BioLegend Cat. No. 511402)\u003cbr\u003e\nStructure: CXC chemokine; 8.4 kD (Mammalian)\u003cbr\u003e\nLigand\/Receptor: CXCR1 (CDw128); CXCR2\u003cbr\u003e\nBioactivity: Neutrophil\/basophil chemoattractant; activates neutrophils, angiogenic factor\u003cbr\u003e\nCell Sources: Monocytes, lymphocytes, granulocytes, fibroblasts, endothelial cells, bronchial epithelial cells, keratinocytes, melanocytes, hepatocytes, mesangial cells, chondrocytes\u003cbr\u003e\nCell Targets: Neutrophils, basophils, lymphocytes\u003cbr\u003e\nCell Type: Neutrophils, Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Baggiolini M, et al. 1994. Adv. Immunol. 55:97. 3. Schröder J, et al. 1992. Immunology Ser. 57:387. 4. Zwahlen R, et al. 1993. Intl. Rev. Expt. Pathol. 34:27.\u003cbr\u003e\nRegulation: Upregulated by IL-1, IL-7, IL-17, IFN-γ, TNF-α, leukoregulin; downregulated by IL-4, TGF-ß, glucocorticoids, vitamin D3\u003cbr\u003e\nGene ID: 3576\u003cbr\u003e\nUniProt: View information about IL-8 on UniProt.org\u003cbr\u003e\nClone: E8N1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interleukin-8, Neutrophil activating protein-1, Monocyte-derived neutrophil chemotactic factor (MDNCF), Neutrophil activating factor (NAF), Leukocyte adhesion inhibitor (LAI), Granulocyte chemotactic protein (GCP), CXCL8\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866088951977,"sku":"511401","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-511401","provider":"Iright","version":"1.0","type":"link"}