{"product_id":"biolegend-511412","title":"Biolegend, 511412, Alexa Fluor® 488 anti-human IL-8 Antibody, 100tests","description":"\u003cp\u003eIL-8, also known as neutrophil chemotactic factor, neutrophil activating protein, and monocyte-derived neutrophil chemotactic factor, is a member of the alpha (C-X-C) subfamily of chemokines called CXCL8. In response to proinflammatory stimuli, IL-8 is produced by monocytes, macrophages, T cells, neutrophils, and fibroblasts. IL-8 promotes neutrophil chemotaxis and degranulation. The 72 amino acid IL-8 is the predominant form secreted by monocytes and lymphocytes. The E8N1 antibody recognizes the human IL-8 protein and has been shown to be useful for intracellular immunofluorescence flow cytometric analysis and as ELISA detection antibody.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: ELISA Detection: The biotinylated E8N1 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified H8A5 (Cat. No. 511502) antibody as the capture antibody. Note: For testing human IL-8 in serum or plasma, BioLegend's ELISA Max™ Sets are specially developed and recommended.\u003cbr\u003e\nProduct Citations: Nizsalóczki E, et al. 2014. Chemphyschem. 3969:15. PubMed Billing F, et al. 2021. ACS Appl Mater Interfaces. 13:55534. PubMed Risnik D, et al. 2017. Sci Rep. 7:15714. PubMed Kim MY, et al. 2018. Cell. 173:1439. PubMed Glaser K, et al. 2016. PLoS One. 11: 0146898. PubMed\u003cbr\u003e\nRRID: AB_893466 (BioLegend Cat. No. 511411) AB_893461 (BioLegend Cat. No. 511412)\u003cbr\u003e\nStructure: CXC chemokine; 8.4 kD (Mammalian)\u003cbr\u003e\nLigand\/Receptor: CXCR1 (CDw128); CXCR2\u003cbr\u003e\nBioactivity: Neutrophil\/basophil chemoattractant; activates neutrophils, angiogenic factor\u003cbr\u003e\nCell Sources: Monocytes, lymphocytes, granulocytes, fibroblasts, endothelial cells, bronchial epithelial cells, keratinocytes, melanocytes, hepatocytes, mesangial cells, chondrocytes\u003cbr\u003e\nCell Targets: Neutrophils, basophils, lymphocytes\u003cbr\u003e\nCell Type: Neutrophils, Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Baggiolini M, et al. 1994. Adv. Immunol. 55:97. 3. Schröder J, et al. 1992. Immunology Ser. 57:387. 4. Zwahlen R, et al. 1993. Intl. Rev. Expt. Pathol. 34:27.\u003cbr\u003e\nRegulation: Upregulated by IL-1, IL-7, IL-17, IFN-γ, TNF-α, leukoregulin; downregulated by IL-4, TGF-ß, glucocorticoids, vitamin D3\u003cbr\u003e\nGene ID: 3576\u003cbr\u003e\nUniProt: View information about IL-8 on UniProt.org\u003cbr\u003e\nClone: E8N1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interleukin-8, Neutrophil activating protein-1, Monocyte-derived neutrophil chemotactic factor (MDNCF), Neutrophil activating factor (NAF), Leukocyte adhesion inhibitor (LAI), Granulocyte chemotactic protein (GCP), CXCL8\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866088427689,"sku":"511412","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-511412","provider":"Iright","version":"1.0","type":"link"}