{"product_id":"biolegend-512503","title":"Biolegend, 512503, Biotin anti-mouse IL-1α Antibody, 50μg","description":"\u003cp\u003eIL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1α is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: E. coli - expressed, recombinant mouse IL-1α\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: ELISA Detection, ELISPOT Detection - Quality testedWB, IP - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.05 - 0.2 µg\/ml is recommended. To obtain a linear standard curve, serial dilutions of mouse IL-1α recobinant protein ranging from 250 to 4 pg\/ml are recommended for each ELISA plate. For Western Blotting applications, it is suggested to use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: ELISA Detection: The biotinylated BL1a-89 antibody is useful as a detection antibody for a sandwich ELISA when used in conjunction with purified BL1a-72 antibody (Cat. No. 512402) or purified ALF-161 antibody (Cat. Nos. 503202 \u0026amp; 503206) as the capture antibody and recombinant mouse IL-1a (Cat. No. 580309) as the standard.\u003cbr\u003e\nRRID: AB_2295879 (BioLegend Cat. No. 512503) AB_2124220 (BioLegend Cat. No. 512504)\u003cbr\u003e\nStructure: Cytokine; 17.4 kD (Mammalian)\u003cbr\u003e\nBioactivity: Stimulates T cells, B cells, proliferation\/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia; monocyte transition from IL-1β to IL-1α when matured to macrophages\u003cbr\u003e\nCell Sources: Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma,\u003cbr\u003e\nCell Targets: B cells, T cells, monocytes, fibroblasts\u003cbr\u003e\nReceptors: Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York. 3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241. 4. Dinarello C. 1996. Blood 87:2095.\u003cbr\u003e\nRegulation: Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin\u003cbr\u003e\nGene ID: 16175\u003cbr\u003e\nUniProt: View information about IL-1alpha on UniProt.org\u003cbr\u003e\nClone: BL1a-89\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interleukin-1α, Lymphocyte-activating factor (LAF), Endogenous pyrogen (EP), Leukocyte endogenous mediator (LEM), Mononuclear cell factor (MCF\/MNCF) (il-1a,il1a)\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866085314729,"sku":"512503","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-512503","provider":"Iright","version":"1.0","type":"link"}