{"product_id":"biolegend-514703","title":"Biolegend, 514703, Biotin anti-human IL-8 Antibody, 50μg","description":"\u003cp\u003eIL-8, also known as neutrophil chemotactic factor, neutrophil activating protein, and monocyte-derived neutrophil chemotactic factor, is a member of the alpha (C-X-C) subfamily of chemokines called CXCL8. In response to proinflammatory stimuli, IL-8 is produced by monocytes, macrophages, T cells, neutrophils, and fibroblasts. IL-8 promotes neutrophil chemotaxis and degranulation. The 72 amino acid IL-8 is the predominant form secreted by monocytes and lymphocytes. The E8N1 antibody recognizes the human IL-8 protein and has been shown to be useful for intracellular immunofluorescence flow cytometric analysis and as ELISA detection antibody.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant human IL-8, amino acids Ser28-Ser99 (Accession # NM_000584)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: ELISA\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.5-2.0 µg\/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-8 recombinant protein ranging from 2000 to 15 pg\/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: ELISA Detection: The biotinylated BH0840 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified BH0814 (Cat. No. 514601\/ 514602) antibody as the capture antibody and recombinant human IL-8 (Cat. # 570909) as the standard.\u003cbr\u003e\nProduct Citations: Korkusuz P, et al. 2018. Methods Mol Biol. 153:1879. PubMed\u003cbr\u003e\nRRID: AB_2126229 (BioLegend Cat. No. 514704)\u003cbr\u003e\nStructure: CXC chemokine; 8.4 kD (Mammalian)\u003cbr\u003e\nLigand\/Receptor: CXCR1 (CDw128); CXCR2\u003cbr\u003e\nBioactivity: Neutrophil\/basophil chemoattractant; activates neutrophils, angiogenic factor\u003cbr\u003e\nCell Sources: Monocytes, lymphocytes, granulocytes, fibroblasts, endothelial cells, bronchial epithelial cells, keratinocytes, melanocytes, hepatocytes, mesangial cells, chondrocytes\u003cbr\u003e\nCell Targets: Neutrophils, basophils, lymphocytes\u003cbr\u003e\nReceptors: CXCR1 (CDw128); CXCR2\u003cbr\u003e\nBiology Area: Cell Biology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Baggiolini M, et al. 1994. Adv. Immunol. 55:97. 3. Schröder J, et al. 1992. Immunology Ser. 57:387. 4. Zwahlen R, et al. 1993. Intl. Rev. Expt. Pathol. 34:27.\u003cbr\u003e\nRegulation: Upregulated by IL-1, IL-7, IL-17, IFN-γ, TNF-α, leukoregulin; downregulated by IL-4, TGF-β, glucocorticoids, vitamin D3\u003cbr\u003e\nGene ID: 3576\u003cbr\u003e\nUniProt: View information about IL-8 on UniProt.org\u003cbr\u003e\nClone: BH0840\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interleukin-8, Neutrophil activating protein-1, Monocyte-derived neutrophil chemotactic factor (MDNCF), Neutrophil activating factor (NAF), Leukocyte adhesion inhibitor (LAI), Granulocyte chemotactic protein (GCP), CXCL8\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866080825513,"sku":"514703","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-514703","provider":"Iright","version":"1.0","type":"link"}