{"product_id":"biolegend-515801","title":"Biolegend, 515801, Biotin anti-mouse IL-1β Antibody, VO19","description":"\u003cp\u003eIL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1β is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. The Poly5158 antibody reacts with the precursor and mature secreted forms of mouse IL-1β.\u003cbr\u003e\nVO19\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Polyclonal\u003cbr\u003e\nHost Species: Goat\u003cbr\u003e\nImmunogen: Recombinant mouse IL-1β\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: ELISA Detection - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection, the suggested use of this reagent is 1:200 dilution. To obtain a linear standard curve, serial dilutions of IL-1β recombinant protein ranging from 2000 to 30 pg\/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: ELISA Detection: The biotinylated Poly5158 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified B122 antibody (Cat. No. 503502) as the capture antibody.\u003cbr\u003e\nAdditional Product Notes: This antibody can be used for 5 plates.\u003cbr\u003e\nProduct Citations: Morita M, et al. 2021. Heliyon. 7:e06228. PubMed Herrada AA, et al. 2022. Front Immunol. 13:841641. PubMed Zhao L, et al. 2021. STAR Protocols. 2:100732. PubMed Pahari S, et al. 2020. Autophagy. 1.375694444. PubMed\u003cbr\u003e\nRRID: AB_2124615 (BioLegend Cat. No. 515801)\u003cbr\u003e\nStructure: Cytokine; 17.5 kD (Mammalian)\u003cbr\u003e\nBioactivity: Stimulates T cells, B cells, proliferation\/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia; monocyte transition from IL-1β to IL-1α when matured to macrophages\u003cbr\u003e\nCell Sources: Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma\u003cbr\u003e\nCell Targets: B cells, T cells, monocytes\u003cbr\u003e\nReceptors: Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York. 3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241. 4. Dinarello C et al. 1996. Blood 87:2095.\u003cbr\u003e\nRegulation: Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin\u003cbr\u003e\nGene ID: 3553\u003cbr\u003e\nUniProt: View information about IL-1beta on UniProt.org\u003cbr\u003e\nClone: Poly5158\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interleukin-1β, Catabolin, Hematopoietin-1 (H1), IFNβ-inducing factor, Interleukin-β, Osteoclast activating factor (OAF)\u003cbr\u003e\nIsotype: Goat Polyclonal IgG\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866162942121,"sku":"515801","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-515801","provider":"Iright","version":"1.0","type":"link"}