{"product_id":"biolegend-517501","title":"Biolegend, 517501, Purified anti-mouse IL-22 Antibody, 25μg","description":"\u003cp\u003eIL-22, a cytokine structurally related to IL-10, was originally identified in the mouse as a gene induced by IL-9 in T cells and mast cells. IL-22 was initially designated as IL-TIF (IL10-related T cell-derived inducible factor). Mouse IL-TIF consists of 179 amino acids, including four cysteins, and has a predicted molecular weight of 20 kD.  Biological activity of IL-22 is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). Both chains of the IL-22R complex belong to the class II cytokine receptor families. Two types of IL-22-binding receptor have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 is produced by immune cells and acts on non-immune cells to regulate local tissue inflammation. As a product of the recently identified T helper 17 lineage of CD4(+) effector lymphocytes, IL-22 plays a critical role in mucosal immunity as well as in dysregulated inflammation observed in autoimmune diseases.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse, Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mature recombinant protein mouse Interleukin-22 (Leu34-Val179)\u003cbr\u003e\nFormulation: This antibody is provided in phosphate-buffered solution, pH 7.2, 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, store undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.5 µg\/ml dilution buffer for each mini-gel.\u003cbr\u003e\nApplication Notes: Clone BL35175 has been shown to be useful for detecting mouse and human IL-22 by Western blotting.\u003cbr\u003e\nProduct Citations: Ogita T, et al. 2021. Front Nutr. 8:701466. PubMed Ke Y, et al. 2011. J Immunol. 187:2130. PubMed Jiao S, et al. 2020. Cell. 179(5):1177-1190.e13.. PubMed\u003cbr\u003e\nRRID: AB_10695475 (BioLegend Cat. No. 517501) AB_10694243 (BioLegend Cat. No. 517502)\u003cbr\u003e\nStructure: 179 aa with with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein.\u003cbr\u003e\nDistribution: Secreted, Th17\u003cbr\u003e\nFunction: Cytokine that contributes to the inflammatory response in vivo.\u003cbr\u003e\nLigand\/Receptor: Binds to IL-22 receptor complex that consists of two receptor subunits, IL22R and IL10Rβ.\u003cbr\u003e\nCell Type: Th17\u003cbr\u003e\nBiology Area: Cell Biology, Signal Transduction\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Dumoutier L, et al. 2000. J. Immunol. 164:1814. 2. Xie MH, et al. 2000. J. Biol. Chem. 275:31335. 3. Jones BC, et al. 2008. Structure 16:1333. 4. Sonnenberg GF, et al. 2010. J. Exp. Med. 207:1293. 5. Kotenko SV, et al. 2001. J. Immunol. 166:7096. 6. Chang C, et al. 2006. Cell Research 16:902.\u003cbr\u003e\nGene ID: 50929\u003cbr\u003e\nUniProt: View information about IL-22 on UniProt.org\u003cbr\u003e\nClone: BL35175\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interleukin-22, IL-TIF, IL-10-related T-cell-derived-inducible factor\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866085511337,"sku":"517501","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-517501","provider":"Iright","version":"1.0","type":"link"}