{"product_id":"biolegend-521003","title":"Biolegend, 521003, Biotin anti-human CCL8 (MCP-2) Antibody, 50μg","description":"\u003cp\u003eCCL8, also known as MCP-2, is a beta chemokine initially isolated from human MG-63 osteosarcoma cells that are stimulated with IL-1β. CCL8 (MCP-2) has a structure and function that is similar with both MCP-1 and MCP-3. It is often co-induced with those chemokines in mononuclear cells. IFNγ is a strong inducer of CCL8 (MCP-2) in monocytes and fibroblasts. CCL8 (MCP-2) in monocytes and fibroblasts and can be processed and secreted as a truncated chemokine CCL8 (6-75). This isoform does not chemo attract monocytes, but it binds to the CCR2 receptor and induces its internalization. As a result, CCL8 (MCP-2) inhibits MCP-1 (CCL2) and MCP-2 (CCL8) ERK signaling and antagonizes the chemotactic activity of several CCR2 ligands (MCP-1\/CCL2, MCP-2\/CCL8, MCP-3\/CCL7). CCL8 (MCP-2) is highly expressed in the skin, and it serves as an agonist for the chemokine receptor CCR8, but not for CCR2. Therefore, it has been speculated that the CCL8-CCR8 interaction is a crucial regulator of the Th2 homing, which drives IL-5 mediated chronic allergic inflammation. In addition, CCL8 (MCP-2) binds the CCR5 receptor with a high affinity and inhibits the replication of human immunodeficiency virus type 1. CCL8 (MCP-2) is highly expressed in different human diseases, and it is a potential biomarker for the diagnosis of graft-versus-host diseases and tuberculosis.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Polyclonal\u003cbr\u003e\nHost Species: Goat\u003cbr\u003e\nImmunogen: Recombinant human CCL8.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: ELISA Detection - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.25 – 1.0 µg\/ml is recommended. To obtain a linear standard curve, serial dilutions of CCL8 (MCP-2) recombinant protein ranging from 250 to 3.9 pg\/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: ELISA Detection: The biotinylated Poly5210 antibody is useful as the detection antibody for a sandwich ELISA assay, when used in conjunction with the purified L3715B6 antibody as the capture antibody and recombinant human CCL8 (MCP-2) as the standard.Note: For testing human CCL8 (MCP-2) in serum, plasma, or supernatant, BioLegend’s LEGEND MAX™ Human CCL8 (MCP-2) ELISA Kit is specially developed and recommended.\u003cbr\u003e\nRRID: AB_2565224 (BioLegend Cat. No. 521003)\u003cbr\u003e\nStructure: Chemokine.\u003cbr\u003e\nDistribution: Fibroblasts, endothelial cells, monocytes, and macrophages.\u003cbr\u003e\nFunction: CCL8 (MCP-2) chemo attracts monocytes, T cells, natural killer (NK) cells, mast cells, eosinophils, and basophils. CCL8 (MCP-2) is induced by IL-1β, IFNβ, IFNγ, dsRNA, virus, ConA, and anti-CD2. The induction of CCL8 (MCP-2) by IFNγ synergizes with the TLR ligands peptidoglycan (TLR2), dsRNA (TLR3), and LPS (TLR4).\u003cbr\u003e\nInteraction: Monocytes, T cells, natural killer (NK) cells, mast cells, eosinophils, and basophils.\u003cbr\u003e\nLigand\/Receptor: CCR1 (CD191), CCR2 (CD192), CCR3 (CD193), and CCR5 (CD195).\u003cbr\u003e\nCell Type: Fibroblasts, Endothelial cells, Monocytes, Macrophages\u003cbr\u003e\nBiology Area: Cell Biology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Van Damme J, et al. 1992. J. Exp. Med. 176:59.2. Proost P, et al. 1998. J. Immunol. 160:4034.3. Dean RA, et al. 2008. Blood 112:3455.4. Hori T, et al. 2008. Blood 111:4403.5. Rom S, et al. 2010. FASEB J. 24:2292.6. Struyf S, et al. 2009. Eur. J. Immunol. 39:843.7. Islam SA, et al. 2011. Nat. Immunol. 12:167.\u003cbr\u003e\nRegulation: Monocytes, T cells, natural killer (NK) cells, mast cells, eosinophils, and basophils.\u003cbr\u003e\nGene ID: 10635\u003cbr\u003e\nUniProt: View information about CCL8 on UniProt.org\u003cbr\u003e\nClone: Poly5210\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: HC14, MCP-2\u003cbr\u003e\nIsotype: Goat Polyclonal IgG\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866164678825,"sku":"521003","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-521003","provider":"Iright","version":"1.0","type":"link"}