{"product_id":"biolegend-526104","title":"Biolegend, 526104, Biotin anti-human Granulysin Antibody, 500μg","description":"\u003cp\u003eGranulysin is a cytolytic protein present in granules of human natural killer (NK) cells and activated cytotoxic T lymphocytes (CTLs). Granulysin is synthesized as a 15 kD precursor, which is then cleaved at both the amino- and carboxy-termini into a 9 kD activated form.  The 9 kD granulysin is released into the intercellular space between target and effector cells via a granule exocytosis pathway. The 9 kD granulysin is a chemoattractant for T lymphocytes, monocytes, and other inflammatory cells and activates the expression of a number of cytokines, including RANTES, MCP-1, MCP-3, MIP-1α, IL-1, IL-6, IL-10 and IFN-α.  High serum levels of granulysin are associated with cytotoxic T cell and NK cell activity, and may thus represent a soluble biomarker for host-cellular immune responses. Serum granulysin has also been proposed as a biomarker for the Th1\/Th2 balance. At the cellular level, granulysin may serve as a favorable prognostic marker for certain types of cancer. \u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant 9 kD granulysin\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: ELISA Detection - Quality testedNeut, IP - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by ELISA assay. A concentration of 1 µg\/µl of the detection antibody was utilized to gernerate the example standard curve. It is recommended that the end-user titrate each lot of reagents for optimal performance.\u003cbr\u003e\nApplication Notes: ELISA Detection: To measure human granulysin, this antibody can be used as a detection antibody in sandwich ELISA format and paired with the purified DH2 antibody (Cat. No. 348007) as the capture antibody.Additional reported applications (for the relevant formats) include: neutralization1 and immunoprecipitation1.Note: For testing human granulysin in serum, plasma or cell culture supernatant, LEGEND MAX™ Human Granulysin ELISA Kit with Pre-coated Plates (Cat. No. 438007 \u0026amp; 438008) are specially developed and recommended.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Tewari P, et al. 2010. Blood. 116:3465. (Neut, IP)\u003cbr\u003e\nRRID: AB_2563603 (BioLegend Cat. No. 526103) AB_2563604 (BioLegend Cat. No. 526104)\u003cbr\u003e\nStructure: Saposin-like protein family of lipid binding protein, 9 kD and 15 kD\u003cbr\u003e\nDistribution: NK cells, cytolytic T cells\u003cbr\u003e\nFunction: Cytolytic against bacteria, fungi, parasite and tumor cells; chemoattract inflammatory cells\u003cbr\u003e\nCell Type: Neutrophils, NK cells, T cells\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Immunology\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Hanson DA, et al. 1999. Mol. Immunol. 36:413. 2. Spada FM, et al. 2000. J. Exp. Med. 191:937. 3. Kalb ML, et al. 2012. J. Immunol. 188:1583.\u003cbr\u003e\nGene ID: 10578\u003cbr\u003e\nUniProt: View information about Granulysin on UniProt.org\u003cbr\u003e\nClone: DH10\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: GNLY, 519, LAG2, NKG5, LAG-2, D2S69E, TLA519\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46863008956585,"sku":"526104","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-526104","provider":"Iright","version":"1.0","type":"link"}