{"product_id":"biolegend-570206","title":"Biolegend, 570206, Recombinant Human IFN-γ (carrier-free), 100μg","description":"\u003cp\u003eInterferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.\u003cbr\u003e\n100μg\u003cbr\u003e\nSource: Human IFN-γ, amino acids Gln24-Gln166 (Accession # NM_000619) was expressed in E. coli.\u003cbr\u003e\nMolecular Mass: The 144 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 16,907 Da. The DTT-reduced protein and the non-reduced protein migrate at approximately 17 kDa by SDS-PAGE.\u003cbr\u003e\nPurity: Purity is \u0026gt;95%, as determined by Coomassie stained SDS-PAGE.\u003cbr\u003e\nFormulation: 0.22 µm filtered protein solution is in 10mM Sodium succinate, 4% Mannitol, pH 5.0.\u003cbr\u003e\nEndotoxin Level: Endotoxin level is \u0026lt;0.1 EU\/µg (\u0026lt;0.01ng\/µg) protein as determined by the LAL method.\u003cbr\u003e\nConcentration: 10 and 25 µg sizes are bottled at 100 µg\/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.\u003cbr\u003e\nStorage \u0026amp; Handling: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg\/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze\/thaw cycles.\u003cbr\u003e\nActivity: Recombinant human IFN-γ induced cytotoxic effect on HT-29 cells in a dose-dependent manner. The ED50 is 1 – 6 ng\/mL.\u003cbr\u003e\nApplication: Bioassay\u003cbr\u003e\nRecommended Usage: Use when high specific biological activity is required.\u003cbr\u003e\nApplication Notes: BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Meissner TB, et al. 2012. J. Immunol. 188:4951. PubMed Meissner TB, et al. 2010. Proc. Natl. Acad. Sci. USA 107:13794. PubMed\u003cbr\u003e\nProduct Citations: Mavrikaki M, et al. 2022. Nat Aging. 2:1130. PubMed Arnold KA, et al. 2023. Int J Mol Sci. 24:. PubMed Jiang Z, et al. 2021. J Clin Invest. 131: . PubMed Zhang Z, et al. 2022. Cancer Gene Ther. 29:722. PubMed Sugawara E, et al. 2020. Autophagy. 1.323611111. PubMed Gruber C, et al. 2020. J Exp Med. 217:00:00. PubMed Nguyen DJM, et al. 2020. Mol Cancer Res. 0.822916667. PubMed Gilad Y, et al. 2019. Sci Rep. 9:15099. PubMed Tsai M, et al. 2022. J Biol Chem. :102698. PubMed Zhang C, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed Stringhini M, et al. 2021. MAbs. 13:1868066. PubMed Rajan SS, et al. 2020. Oncogene. 39:2103. PubMed Zhang H, et al. 2020. Front Cell Dev Biol. 8:205. PubMed Tran L, et al. 2017. Cancer Immunol Res. 5:1141. PubMed Nenasheva T, et al. 2020. Front Immunol. 1.163888889. PubMed Baleeiro RB, et al. 2022. Oncoimmunology. 11:2080329. PubMed Guo J, et al. 2019. Cancer Immunol Res. 1.349305556. PubMed Rafieerad A, et al. 2021. Adv Funct Mater. 31:2106786. PubMed Taniguchi H, et al. 2022. Cell Rep. 39:110814. PubMed Lin H, et al. 2020. Small. 16:e2002194. PubMed Becker W, et al. 2021. J Crohns Colitis. 15:1032. PubMed Yamaguchi Y, et al. 2022. J Immunother Cancer. 10:. PubMed Brasil da Costa FH, et al. 2020. PLoS One. 15:e0230354. PubMed Sharma V, et al. 2021. ACR Open Rheumatol. 3:277. PubMed Wrenn ED, et al. 2020. Cell. 183:395. PubMed Miyamoto T, et al. 2021. Cancer Immunol Res. Online ahead of print. PubMed Shikama Y, et al. 2022. Inflammation. . PubMed Guo Q, et al. 2022. Blood Adv. 6:5668. PubMed Stebbing J, et al. 2021. Sci Adv. 7:00. PubMed Jenks SA et al. 2018. Immunity. 49(4):725-739 . PubMed Chen B, et al. 2021. Cell. 184:6262. PubMed Hogg SJ, et al. 2022. Clin Epigenetics. 14:96. PubMed\u003cbr\u003e\nStructure: Cytokine; dimer\u003cbr\u003e\nBioactivity: Antiviral\/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC\u003cbr\u003e\nCell Sources: CD8+ and CD4+ T cells, NK cells\u003cbr\u003e\nCell Targets: T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts\u003cbr\u003e\nReceptors: IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)\u003cbr\u003e\nCell Type: Embryonic Stem Cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Stem Cells\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321. 3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571. 4. Gray P, et al. 1987. Lymphokines 13:151.\u003cbr\u003e\nRegulation: Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2\u003cbr\u003e\nGene ID: 3458\u003cbr\u003e\nUniProt: View information about IFN-gamma on UniProt.org\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF),\u003cbr\u003e\nGMP Version Available: View Product\u003cbr\u003e\nQ: Why choose BioLegend recombinant proteins?\u003cbr\u003e\nA: • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.\u003cbr\u003e\n     • Greater than 95% Purity or higher, tested on every lot of product.\u003cbr\u003e\n     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.\u003cbr\u003e\n     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.\u003cbr\u003e\n     • Bulk and customization available. Contact us.\u003cbr\u003e\n     • Learn more about our Recombinant Proteins.\u003cbr\u003e\nQ: How does the activity of your recombinant proteins compare to competitors?\u003cbr\u003e\nA: We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!\u003cbr\u003e\nQ: What is the specific activity or ED50 of my recombinant protein?\u003cbr\u003e\nA: The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells\/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.\u003cbr\u003e\nQ: Have your recombinants been tested for stability?\u003cbr\u003e\nA: Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.\u003cbr\u003e\nQ: Does specific activity of a recombinant protein vary between lots?\u003cbr\u003e\nA: Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.\u003cbr\u003e\nQ: How do you convert activity as an ED50 in ng\/ml to a specific activity in Units\/mg?\u003cbr\u003e\nA: Use formula Specific activity (Units\/mg) = 10^6\/ ED50 (ng\/mL)\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864321544361,"sku":"570206","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-570206","provider":"Iright","version":"1.0","type":"link"}