{"product_id":"biolegend-603252","title":"Biolegend, 603252, Purified anti-PGK1 Antibody, 100μg","description":"\u003cp\u003ePhosphoglycerate kinase 1 (PGK1) is one of two enzymes, along with its isozyme PGK2, which catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, and transfers the phosphate group to ADP to produce ATP. This step is notable as the “break even” point of glycolysis where ATP expenditure matches ATP output. Additionally, PGK1 functions as a protein kinase, and is also involved in transcription initiation as a cofactor for DNA polymerase alpha. The gene encoding PGK1 is on the X chromosome, and mutations in PGK1 are associated with various cancers, and X-linked recessive variants of anemia, mental disorders, and myopathy.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial fragment of recombinant Human PGK1\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested IHC-P, ICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1 µg\/mL. For immunohistochemistry, a concentration range of 1 - 10 µg\/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 5 μg\/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nRRID: AB_2910481 (BioLegend Cat. No. 603251) AB_2910481 (BioLegend Cat. No. 603252)\u003cbr\u003e\nStructure: PGK1 is a 417 amino acid protein with a predicted molecular weight of 44.6 kD.\u003cbr\u003e\nDistribution: PGK1 is widely expressed in the cytoplasm of most tissues.\u003cbr\u003e\nFunction: Enzyme catalyzing the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Protein kinase.\u003cbr\u003e\nInteraction: PDHK1\u003cbr\u003e\nBiology Area: Cancer Biomarkers\u003cbr\u003e\nMolecular Family: Enzymes and Regulators, Protein Kinases\/Phosphatase\u003cbr\u003e\nAntigen References: Fu Q and Yu Z. 2020. Life Sci. 256:117863.\u003cbr\u003e\nGene ID: 5230\u003cbr\u003e\nUniProt: View information about PGK1 on UniProt.org\u003cbr\u003e\nClone: W18295A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Phosphoglycerate Kinase 1 (PGK1), cell migration-inducing gene 10 protein, primer recognition protein 2 (PRP2), Epididymis Secretory Sperm Binding Protein Li 68p, EC 2.7.2.3\u003cbr\u003e\nIsotype: Rat IgG2a, λ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866338775209,"sku":"603252","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-603252","provider":"Iright","version":"1.0","type":"link"}