{"product_id":"biolegend-603351","title":"Biolegend, 603351, Purified anti-RIP-1 Antibody, 25μg","description":"\u003cp\u003eReceptor-interacting serine\/threonine-protein kinase 1 (RIP-1) is a member of the RIP family of serine\/threonine kinases and consists of a kinase domain (KD) at its N-terminus, and a death domain (DD) in its C-terminus. It is a key regulator of cellular inflammatory response and cell survival when functioning as a scaffold protein, namely as part of the NF-κB signaling pathway, and is a key regulator of cell death when functioning as a kinase. RIP-1 promotes cell survival as part of the tumor necrosis factor receptor 1 (TNFR1) complex following ubiquitination by M1 and K63, and phosphorylation mediated by IKK. When ubiquitination is disrupted by ubiquitin E3 ligases, RIP-1 associates with FADD and caspase 8 to promote apoptosis or forms a complex with RIPK3 to induce necroptosis. Due to it being part of the TNF signaling pathway and its dual roles in cell survival and cell death, RIP-1 is of particular interest as a therapeutic target, namely as a mediator in the induction of immunogenic cell death and the treatment of cancer as well as in the treatment of autoimmune and neurodegenerative disorders.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial recombinant human RIP1 protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 µg\/mL. For immunocytochemistry, a concentration of 5 μg\/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This clone was tested for ICC using HeLa cells with localization expected in the cytosol and plasma membrane. Three fix\/perm methods were used: methanol fixation, or 4% PFA fixation followed by permeabilization with either methanol or Triton X-100. Methanol-only fixation produced a strong signal with the expected cytoplasmic localization. PFA-fixation followed by permeabilization with either methanol or Triton X-100 did not produce the expected cytoplasmic\/membrane signal. This clone was tested for IHC-P on human skin tissue using Biolegend’s AEC Chromagen. Positive signal was not observed.\u003cbr\u003e\nRRID: AB_2922634 (BioLegend Cat. No. 603351) AB_2922634 (BioLegend Cat. No. 603352)\u003cbr\u003e\nStructure: RIP-1 is a 671 amino acid protein with a predicted molecular weight of 76 kD.\u003cbr\u003e\nDistribution: Ubiquitously expressed\/localized to the cell membrane and cytoplasm\u003cbr\u003e\nFunction: Serine\/threonine-protein kinase, Transferase\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Cell Death, Neurodegeneration, Neuroscience\u003cbr\u003e\nMolecular Family: Protein Kinases\/Phosphatase\u003cbr\u003e\nAntigen References: Ang RL, et al. 2019. Front Cell Dev Biol. 7:163. Degterev A, et al. 2019. Proc Natl Acad Sci U S A. 116:9714-22. Moriwaki K, et al. 2021. Mucosal Immunol. 15:84-95. Smith HG, et al. 2020. EMBO Mol Med. 12:e10979.\u003cbr\u003e\nGene ID: 8737\u003cbr\u003e\nUniProt: View information about RIP-1 on UniProt.org\u003cbr\u003e\nClone: W17101A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Receptor-Interacting Protein Kinase 1, RIP, RIP-1, RIP1, IMD57, AIEFL, Receptor-Interacting Serine\/Threonine-Protein Kinase\u003cbr\u003e\nIsotype: Rat IgG2b, λ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866313052329,"sku":"603351","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-603351","provider":"Iright","version":"1.0","type":"link"}