{"product_id":"biolegend-605051","title":"Biolegend, 605051, Purified anti-APEX1 Antibody, 25μg","description":"\u003cp\u003eApurinic-apyridimic endonuclease-1, or APEX1, is a multifunctional enzyme required for the repair of DNA damaged by both endogenous and exogenous agents. It also exerts oncogenic function as a redox signaling protein by reductively stimulating the DNA-binding activity of transcription factors that drive cancer progression, including c-Jun, AP-1, NF- κ B, p53, and HIF1α. APEX1 expression level and subcellular localization are positively correlated with cancer progression, and elevated APEX1 expression may also confer resistance to DNA-damaging chemotherapeutic agents.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Full-length recombinant human APEX1 protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested IHC-P, ICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg\/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration of 5.0 µg\/mL is suggested. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg\/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: When using this clone for ICC, we recommend using PFA + methanol or methanol only fix-perm conditions. We do not recommend using PFA + Triton X-100 due to non-specific staining. When testing this clone for ICC, this antibody produced filamentous, extranuclear staining in some cells. This pattern was also observed with a control antibody generated using a different APEX1 immunogen, consistent with this staining being APEX1 dependent.\u003cbr\u003e\nRRID: AB_2910484 (BioLegend Cat. No. 605051) AB_2910484 (BioLegend Cat. No. 605052)\u003cbr\u003e\nStructure: APEX1 is a 318 amino acid protein with a predicted molecular weight of ~36 kD.\u003cbr\u003e\nDistribution: Nucleus\/Ubiquitously expressed\u003cbr\u003e\nFunction: DNA repair enzyme\u003cbr\u003e\nBiology Area: Cell Biology, DNA Repair\/Replication\u003cbr\u003e\nMolecular Family: Enzymes and Regulators\u003cbr\u003e\nAntigen References: Fishel ML, et al. 2008. DNA Repair. 7:177-86. Kim MH, et al. 2013. J Clin Invest. 123:3211-3230. Thakur, et al. 2014. Exp Mol Med. 46:e106.\u003cbr\u003e\nGene ID: 328\u003cbr\u003e\nUniProt: View information about APEX1 on UniProt.org\u003cbr\u003e\nClone: 1518CT337.123.86.269.232\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Apurinic\/Apyrimidinic Endodeoxyribonuclease 1, REF1, HAP1, APEN, APE, APX, AP Endonuclease 1\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860811698345,"sku":"605051","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-605051","provider":"Iright","version":"1.0","type":"link"}