{"product_id":"biolegend-607002","title":"Biolegend, 607002, Purified anti-Spt16 (FACT140 complex) Antibody, 100μg","description":"\u003cp\u003eSpt16 is a 120-140 kD nuclear protein and a component of the FACT complex (facilitates chromatin transcription). The FACT140 complex is composed of the Spt16 and the SSRP1 proteins. This complex interacts with nucleosomes and histone H2A\/H2B dimers to promote nucleosome disassembly and allow transcription elongation. The 8D2 monoclonal antibody recognizes human Spt16 and has been shown to be useful for Western blotting.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant (full length)\u003cbr\u003e\nFormulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, store between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC, ChIP - Verified IP - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration range of 1.0 - 5.0 µg per ml is recommended. For ChIP applications, the suggested dilution is 1:50 - 1:100 by volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: 25 µg, 100 µg of Go-ChIP-Grade™ Purified Antibody can be used for 2-4, 11-16 immunoprecipitations, respectively, at the recommended dilution. ICC positive in HeLa Cells.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Tan B C-M, et al. 2006. EMBO doi:10.1038\/sj.emboj.7601271 Adelmant G, et al. 2012. Mol Cell Proteomics. 11:411. PubMed Lolis AA, et al. 2013. J Biol Chem. 288:7676. PubMed Shakya A, et al. 2015. Mol Cell Biol. 35:1014. PubMed\u003cbr\u003e\nProduct Citations: Goswami I, et al. 2022. EMBO Rep. 23:e53684. PubMed Burmistrova D, et al. 2016. PLoS One. 11: 0150958. PubMed Fleyshman D, et al. 2017. Oncotarget. 8:20525. PubMed Adelmant G, et al. 2012. Mol Cell Proteomics. 11:411. PubMed Isa NF, et al. 2021. Vaccines (Basel). 9:. PubMed Safina A, et al. 2017. Nucleic Acids Res. 10.1093\/nar\/gkw1366. PubMed Shakya A, et al. 2015. Mol Cell Biol. 35:1014. PubMed Hu X, et al. 2019. Front Genet. 10:684. PubMed Kaja A, et al. 2021. Mol Cell Biol. 41:. PubMed Lolis A, et al. 2013. J Biol Chem. 288:7676. PubMed Lin D, et al. 2018. EMBO J. 37. PubMed Sandlesh P, et al. 2018. PLoS One. 15:81. PubMed Prendergast L, et al. 2020. Oncogene. 39:5124. PubMed\u003cbr\u003e\nRRID: AB_315688 (BioLegend Cat. No. 607001) AB_315689 (BioLegend Cat. No. 607002)\u003cbr\u003e\nStructure: 120-140 kD\u003cbr\u003e\nDistribution: Nuclear\u003cbr\u003e\nFunction: Interacts with nucleosomes and histone H2A\/H2B dimers to promote nucleosome disassembly and allow transcription elongation\u003cbr\u003e\nInteraction: Component of FACT ATP-dependent chromatin remodeling complex, SSRP1 protein in FACT complex, H2A\/H2B dimers\u003cbr\u003e\nBiology Area: Cell Biology, Chromatin Remodeling\/Epigenetics, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. LeRoy G, et al. 1998. Science 282:1836. 2. Orphanides G, et al. 1998. Cell 92:105. 3. Orphanides G, et al. 1999. Nature 400:284.\u003cbr\u003e\nGene ID: 11198\u003cbr\u003e\nUniProt: View information about Spt16 FACT140 complex on UniProt.org\u003cbr\u003e\nClone: 8D2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Facilitates Chromatin Transcription (FACT140), Spt 16 protein, Cdc68\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862493614249,"sku":"607002","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-607002","provider":"Iright","version":"1.0","type":"link"}