{"product_id":"biolegend-607802","title":"Biolegend, 607802, Purified anti-RUNX1 Antibody, 100μg","description":"\u003cp\u003eRUNX1 belongs to the runt domain family of transcription factors and regulates target gene expression through forming a heterodimeric DNA-binding complex with CBFB. RUNX1 was first identified as a RUNX1-ETO fusion protein in acute myeloid leukaemia (AML) and is frequently mutated in AML and myelodysplastic syndrome due to chromosomal translocation. RUNX1-deficient mice fail to generate hematopoietic stem cells. RUNX1 regulates CD4 gene transcription during multiple stages of T cell development and represses the CD4 gene in CD4-CD8- (double negative) T cells. RUNX1 is also required for the differentiation of CD8+, Th17, and regulatory T cells. Impaired differentiation of megakaryocytes and decreased circulating platelet count was observed in the absence of RUNX1. These findings revealed that RUNX1 acts as a tumor suppressor for myeloid leukemia and is crucial for the development and terminal differentiation of several blood cell lineages.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant human RUNX1 (amino acids 191-453) expressed in E. coli.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:500-1:1000 diluted (0.5 – 1 µg\/mL). It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: In contrast to BioLegend anti-RUNX1 clone 1C5B16, which shows reactivity towards both human and mouse RUNX1, clone W16118A is specific for human RUNX1 and does not cross-react with mouse RUNX1 (please see supplemental data). Clone W16118A (rat host) does not bind RUNX1 as strongly as 1C5B16 (mouse host), but still performed comparably to a competitor’s antibody.This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).\u003cbr\u003e\nRRID: AB_2721748 (BioLegend Cat. No. 607801) AB_2721749 (BioLegend Cat. No. 607802)\u003cbr\u003e\nStructure: 453 amino acids encode a protein with a predicted MW of 48.7 kD. Contains a Runt domain responsible for DNA binding.\u003cbr\u003e\nDistribution: Nucleus\u003cbr\u003e\nFunction: Plays crucial roles in hematopoiesis, hair follicle development, neurogenesis, chondrogenesis, and leukemogenesis.\u003cbr\u003e\nInteraction: Interacts with TLE1, ELF1, ELF2, ELF4, SPI1, KAT6A, KAT6B, YAP1, and CDK6. Forms a heterodimer with CBFB.\u003cbr\u003e\nBiology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Goyama S, et al. 2013. J. Clin. Invest. 123:3876. 2. Wang J, et al. 2013. PLoS One. 18:e74255. 3. Taniuchi I, et al. 2012. EMBO J. 31:4098. 4. Neel BG, et al. 2012. Genes Dev. 26:1520. 5. Della Gatta G, et al. 2012. Nat. Med. 18:436. 6. Hoi CS, et al. 2010. Mol. Cell Biol. 30:2518.\u003cbr\u003e\nGene ID: 861\u003cbr\u003e\nUniProt: View information about RUNX1 on UniProt.org\u003cbr\u003e\nClone: W16118A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: AML1, CBFA2, PEBP2aB, RUNX1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866304303273,"sku":"607802","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-607802","provider":"Iright","version":"1.0","type":"link"}