{"product_id":"biolegend-607902","title":"Biolegend, 607902, Purified anti-GAPDH Antibody, 100μg","description":"\u003cp\u003eGAPDH is well known for its glycolytic function of converting D-glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate. GAPDH is a ubiquitously expressed and has a molecular mass of 36 kD. Though differentially expressed from tissue to tissue, GAPDH is frequently used as a loading control for assays involving mRNA and protein detection. In more recent studies, GAPDH has been shown to be involved in microtubule bundling, prostate cancer progression, programmed neuronal cell death, DNA replication, and DNA repair. Recent work has elucidated roles for GAPDH in apoptosis, gene expression and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant protein (161-335 a.a.)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality testedICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.0375-0.5 µg per ml (1:1000-1:16000 dilution). For immunocytochemistry, a concentration range of 0.25-1.0 μg\/ml (1:500-1:2000 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This clone weakly reacts with rat GAPDH.\u003cbr\u003e\nProduct Citations: Xie Q, et al. 2023. iScience. 26:106395. PubMed Qin XY, et al. 2023. Cell Death Dis. 14:358. PubMed Featherby SJ, et al. 2025. Mol Med Rep. :31. PubMed Su T, et al. 2021. Metabolites. 11:. PubMed Nchioua R, et al. 2022. J Virol. 96:e0059422. PubMed Hayn M, et al. 2021. Cell Reports. 35(7):109126. PubMed Hirschenberger M, et al. 2021. STAR Protoc. 2:100781. PubMed Nchioua R, et al. 2020. MBio. 11:00. PubMed Srinivasachar Badarinarayan S, et al. 2020. Nucleic Acids Res. 48:10890. PubMed Prelli Bozzo C, et al. 2021. Nat Commun. 12:4584. PubMed Bosso M, et al. 2021. Cell Rep. 36:109735. PubMed Bosso M, et al. 2021. STAR Protoc. 2:100236. PubMed Bosso M, et al. 2020. PLoS Pathog. 16:e1008752. PubMed Simon Langer et al. 2019. eLife. 8 pii: e41930. PubMed Stanfield BA, et al. 2021. PLoS One. 16:e0245169. PubMed Nchioua R, et al. 2021. J Virol. 95:e0080821. PubMed Chamberlain N, et al. 2022. Int J Mol Sci. 23:. PubMed Chen HL, et al. 2021. Pharm Biol. 59:134. PubMed Viereckl MJ, et al. 2022. Biomolecules. 12:. PubMed Hopfensperger K, et al. 2020. MBio. 11:00. PubMed Huq S, et al. 2020. Mol Cancer Ther. 19:1797. PubMed Hayashi M, et al. 2021. Viruses. 13:. PubMed Zhao Z, et al. 2021. PLoS Pathog. 17:e1009728. PubMed\u003cbr\u003e\nRRID: AB_2734502 (BioLegend Cat. No. 607901) AB_2734503 (BioLegend Cat. No. 607902)\u003cbr\u003e\nStructure: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 36 kD. The enzyme exists as a tetramer of identical chains.\u003cbr\u003e\nDistribution: Ubiquitously expressed, locate in cytoplasm and perinuclear region.\u003cbr\u003e\nFunction: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 36 kD. The enzyme exists as a tetramer of identical chains.\u003cbr\u003e\nInteraction: Interacts with TPPP. Interacts with EIF1AD and WARS. Interacts with SUMO4, GLUT4, nPKC-iota and CAMK2.\u003cbr\u003e\nBiology Area: Cell Biology, Western Blot Controls\u003cbr\u003e\nAntigen References: Ercolani L, et al. 1988. J. Biol. Chem. 263:15335. Meyer-Siegler K, et al. 1991. Proc. Natl. Acad. Sci. USA. 88:8460. Hara MR, Snyder SH. 2006. Cell. Mol. Neurobiol. 26:527. Zheng L, et al. 2003. Cell. 114:255. Wang Q, et al. 2005. FASEB J. 19:869. Bae BI, et al. 2006. Proc. Natl. Acad. Sci. USA. 103:3405.\u003cbr\u003e\nGene ID: 5590\u003cbr\u003e\nUniProt: View information about GAPDH on UniProt.org\u003cbr\u003e\nClone: W17079A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: glyceraldehyde-3-phosphate dehydrogenase\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866312855721,"sku":"607902","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-607902","provider":"Iright","version":"1.0","type":"link"}