{"product_id":"biolegend-610401","title":"Biolegend, 610401, Purified anti-SMAD1 Phospho (S463\/S465)\/SMAD9 (S465\/S467) Antibody, 25μg","description":"\u003cp\u003eSMAD1 and SMAD9 (SMAD8) are members of SMAD family, whose members play a critical role in signal transduction from the TGF-β receptor kinases. Activation of these receptors results in phosphorylation of SMAD1 and SMAD9 at a C-terminal SSXS motif (Ser463\/Ser465 in SMAD1; Ser465\/Ser467 in SMAD9), leading to dimerization with the coactivator SMAD4. The SMAD1-4 complex is translocated to the nucleus where it induces expression of target genes involved in differentiation, self-renewal, proliferation, and apoptosis.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse, Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Because the sequence of the synthetic phosphopeptide immunogen is completely conserved between SMAD1 and the corresponding residues in SMAD9 (Ser465\/Ser467), this clone is predicted to recognize both phosphorylated proteins. This clone is predicted to recognize rat SMAD1 Phospho (Ser463\/Ser465) and SMAD9 Phospho (Ser465\/Ser467) due to complete sequence homology between the immunizing peptide and each rat homolog.\u003cbr\u003e\nRRID: AB_2801107 (BioLegend Cat. No. 610401) AB_2801108 (BioLegend Cat. No. 610402)\u003cbr\u003e\nStructure: SMAD1 is a 465 amino acid protein with a predicted molecular weight of 52 kD. SMAD9 is 467 amino acid protein with a predicted molecular weight of 52 kD.\u003cbr\u003e\nDistribution: Ubiquitous expression\/cytosolic and nuclear distribution\u003cbr\u003e\nFunction: Transcriptional activation\/TGF- β signaling\u003cbr\u003e\nInteraction: SMAD4, SMURF1, USP15, CBP, p300\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Cell Proliferation and Viability, Signal Transduction, Transcription Factors\u003cbr\u003e\nMolecular Family: Phospho-Proteins\u003cbr\u003e\nAntigen References: Hoodless PA, et al. 1996. Cell. 85: 489-500. Whitman M. 1998. Genes Dev. 12: 2445-62. Alarcon C, et al. 2009. Cell. 139: 757-69.\u003cbr\u003e\nGene ID: 40864093\u003cbr\u003e\nUniProt: View information about SMAD1 Phospho Ser463\/Ser465 SMAD9 Phospho Ser465\/Ser467 on UniProt.org\u003cbr\u003e\nClone: A17019A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Mothers against decapentaplegic homolog 1, JV4-1, Mad-related protein 1\u003cbr\u003e\nIsotype: Mouse IgG1, λ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864404807849,"sku":"610401","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-610401","provider":"Iright","version":"1.0","type":"link"}