{"product_id":"biolegend-610552","title":"Biolegend, 610552, Purified anti-HOMER1 Antibody, 100μg","description":"\u003cp\u003eHOMER1 is a scaffolding protein that is a major component of the post-synaptic density (PSD), a region in the plasma membrane of the post-synaptic neuron that interacts with the pre-synaptic active zone of upstream neurons. HOMER1 consists of two domains, a C-terminal coiled-coil domain that is required for HOMER1 dimerization, and an EVH1 domain involved in protein-protein interaction. Dimerized long-form HOMER1 (variants designated as Homer1b and Homer1c) is constitutively expressed in neurons, where it plays a role in neurotransmitter receptor signaling and trafficking by cross-linking associated proteins via the EVH1 domain. Truncated HOMER1 (Homer1a) is expressed as a result of neuronal activity, and lacking the coiled-coil domain, is incapable of dimerizing and cannot crosslink interacting proteins. Thus, Homer1a acts as a dominant-negative regulator of HOMER1 and is thought to provide negative feedback against excessive neuronal activity.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant human HOMER1\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 0.5 µg\/mL. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This product is not recommended for use in Immunohistochemistry. This product does not recognize mouse or human HOMER1 in Western Blot. This product only recognizes full length HOMER1 (Homer1b\/c) and not the truncated variant Homer1a.\u003cbr\u003e\nRRID: AB_2936737 (BioLegend Cat. No. 610551) AB_2936737 (BioLegend Cat. No. 610552)\u003cbr\u003e\nStructure: HOMER1 is a 354 amino acid protein with a predicted molecular weight of 40 kD.\u003cbr\u003e\nDistribution: Post-synaptic density\u003cbr\u003e\nFunction: Protein-protein interactions, synaptic plasticity\u003cbr\u003e\nInteraction: Homodimer; also interacts with other major components of the PSD including Shank family proteins, cytoskeletal proteins like dynamin, and receptors such as group 1 metabotropic glutamate receptors and the ryanodine receptor.\u003cbr\u003e\nCell Type: Mature Neurons, Neurons\u003cbr\u003e\nBiology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Synaptic Biology\u003cbr\u003e\nMolecular Family: Postsynaptic proteins, Scaffold Proteins\u003cbr\u003e\nAntigen References: Foa L, et al. 2001. Nat Neurosci. 4:499-506. de Bartolomeis A, et al. 2022. Neurosci Biobehav Rev. 136:104596. Salanova M, et al. 2013. IUBMB Life. 65:769-76. Reshetnikov VV, et al. 2021. Biochemistry (Mosc). 86:613-626.\u003cbr\u003e\nGene ID: 9456\u003cbr\u003e\nUniProt: View information about HOMER1 on UniProt.org\u003cbr\u003e\nClone: A18192B\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Homer protein homolog 1 (HOMER1), SYN47\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866063491241,"sku":"610552","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-610552","provider":"Iright","version":"1.0","type":"link"}