{"product_id":"biolegend-611002","title":"Biolegend, 611002, Purified anti-HDAC1 Phospho (Ser406) Antibody, 100μg","description":"\u003cp\u003eHistone Deacetylase 1 (HDAC1) plays a critical role in various cellular processes, including cell cycle progression, proliferation, and differentiation. The enzyme functions by removing acetyl moieties from histone targets, resulting in histone compaction and alterations in nucleosomal positioning.  Aurora kinases phosphorylate HDAC1 at Ser406 during prophase, immediately after cells begin mitosis, resulting in reduced deacetylase activity of HDAC1. This modification plays an essential role in regulating cell cycle progression, as well as controlling the expression of genes involved in central nervous system development.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Zebrafish\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Synthetic peptide corresponding to human HDAC1 phosphorylated at Serine 406\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. For intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: When tested for western blot, this clone produced a band that showed a ~1-3 kD mass shift compared to a pan HDAC1 antibody. This observation is consistent with a previous study of the HDAC1 Phospho (Ser406) site. This clone recognizes zebrafish HDAC1 phosphorylated at Ser4062. Clone BT-15 cross-reacts to Zebrafish2\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Segre CV, et al, 2016. mAbs. 8: 37-42 Loponte S, et al, 2016. Sci Rep, 6: 30213.\u003cbr\u003e\nRRID: AB_2801111 (BioLegend Cat. No. 611001) AB_2801112 (BioLegend Cat. No. 611002)\u003cbr\u003e\nStructure: HDAC1 is a 482 amino acid protein with a predicted molecular weight of 55 kD.\u003cbr\u003e\nDistribution: Nucleus\/Ubiquitously expressed\u003cbr\u003e\nFunction: Phosphorylation of HDAC1 Ser406 results in reduced deactylase activity of the enzyme.\u003cbr\u003e\nBiology Area: Cell Biology, Cell Cycle\/DNA Replication, Chromatin Remodeling\/Epigenetics\u003cbr\u003e\nMolecular Family: Nuclear Markers, Phospho-Proteins\u003cbr\u003e\nAntigen References: Segre CV, et al, 2016. mAbs. 8: 37-42. Loponte S, et al, 2016. Sci Rep, 6: 30213.\u003cbr\u003e\nGene ID: 3065\u003cbr\u003e\nUniProt: View information about HDAC1 Phospho Ser406 on UniProt.org\u003cbr\u003e\nClone: BT-15\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Histone Deactylase 1, RPD3L1\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862917468329,"sku":"611002","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-611002","provider":"Iright","version":"1.0","type":"link"}