{"product_id":"biolegend-612303","title":"Biolegend, 612303, Biotin anti-Cytochrome c Antibody, 100μg","description":"\u003cp\u003eCytochrome c is a 15 kD protein found in the mitochondrial intermembrane space with a heme-binding domain. Cytochrome c is a component of the electron transport chain; the heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Cytochrome c initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9. Cytochrome c interacts with the cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, and Caspase 9 proteins. The 6H2.B4 monoclonal antibody recognizes human, mouse, and rat cytochrome-c and has been shown to be useful for intracellular flow cytometric staining, Western blotting, immunoprecipitation, and immunofluorescence staining.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse, Rat, Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Rat cyt c-OVA\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: intracellular flow cytometry5, immunofluorescence microscopy3,5, immunoprecipitation4, and immunocytochemistry5.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Goshorn SC, et al. 1991. J. Biol. Chem. 266:2134. Jemmerson R, et al. 1991. Eur. J. Immunol. 21:143. Chandra D, et al. 2002. J. Biol. Chem. 277:50842. (IF) Semenkova L, et al. 2003. Eur. J. Biochem. 270:4388. (IP) Shih S-F, et al. 2001. J. Biol. Chem. 276:21870. (ICFC ICC IF) She P, et al. 2011. Am J. Physiol Endcorinol Metab.301:E49. PubMed McGuire, KA., et al. 2011. J. Virol 85:10806. PubMed\u003cbr\u003e\nProduct Citations: Zahno A, et al. 2011. Biochem Pharmacol. 81:432. PubMed Dent JR, et al. 2019. J Appl Physiol (1985). 127:1117. PubMed\u003cbr\u003e\nRRID: AB_315776 (BioLegend Cat. No. 612303)\u003cbr\u003e\nStructure: Heme binding domain; 15 kD\u003cbr\u003e\nDistribution: Mitochondrial intermembrane space\u003cbr\u003e\nFunction: Component of electron transport chain; heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9\u003cbr\u003e\nInteraction: Cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, Casp9\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Mitochondrial Function, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: Mitochondrial Markers\u003cbr\u003e\nAntigen References: 1. Liu X, et al. 1996. Cell. 86:147. 2. Li P, et al. 1997. Cell. 91:479. 3. Zhang Z, et al. 2003. Gene 312:61. 4. Ferguson H, et al. 2003. J. Biol. Chem. 278:45793.\u003cbr\u003e\nGene ID: 1355\u003cbr\u003e\nUniProt: View information about Cytochrome c on UniProt.org\u003cbr\u003e\nClone: 6H2.B4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Cyt c\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862479884457,"sku":"612303","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-612303","provider":"Iright","version":"1.0","type":"link"}