{"product_id":"biolegend-616951","title":"Biolegend, 616951, Purified anti-CD276 (B7-H3) Antibody, 25μg","description":"\u003cp\u003eCD276, or B7-H3, is a type I transmembrane protein and shares 20-27% amino acid identity with other B7 family members. Human CD276 has a single extracellular variable-type immunoglobulin (IgV)-IgC domain and a signature intracellular domain. There also exists an additional isoform known as 4Ig-B7-H3, containing a nearly exact tandem duplication of the IgV-IgC domain. Expression of CD276 is induced on T cells, natural killer (NK) cells, and antigen-presenting cells (APCs), including dendritic cells (DCs) and macrophages, and is upregulated during the maturation of monocytes to DCs, or following interactions between DCs and regulatory T cells. CD276 inhibits T-cell responses, and plays a role in adaptive immunity by suppressing T cell activation. CD276 is also expressed in various cancers, where it works to suppress tumor antigen-specific immune responses.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant fragment of human CD276\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: IHC-P - Quality tested FC, ICC, WB - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg\/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg\/mL is recommended. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg\/mL. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Clone A20079E does not cross-react with mouse CD276 in WB.\u003cbr\u003e\nRRID: AB_3068218 (BioLegend Cat. No. 616951) AB_3068218 (BioLegend Cat. No. 616952)\u003cbr\u003e\nStructure: CD276 is a 534 amino acid protein with a predicted molecular weight of 58.2 kD.\u003cbr\u003e\nDistribution: Dendritic cells, macrophages, natural killer cells, induced T cells. In the tonsil, it is expressed in epithelial cells.\u003cbr\u003e\nFunction: Co-stimulatory molecule that inhibits T-cell responses\u003cbr\u003e\nLigand\/Receptor: CD276 binds to Trem-like transcript 2 (TLT-2).\u003cbr\u003e\nBiology Area: Cell Biology, Costimulatory Molecules, Immunology, Inhibitory Molecules, Innate Immunity\u003cbr\u003e\nAntigen References: Nagashima O, et al. 2008. J Immunol. 181:4062-71. Prasad DVR, et al. 2004. J Immunol. 173:2500-6. Sun M, et al. 2002. J Immunol. 168:6294-7. Xu J, et al. 2006. Cellular and Molecular Immunology. 3:235-40. Ford JW, et al. 2009. Curr Opin Immunol. 21:38-46. Leitner J, et al. 2009. Eur J Immunol. 2009. 39:1754-64.\u003cbr\u003e\nGene ID: 80381\u003cbr\u003e\nUniProt: View information about CD276 on UniProt.org\u003cbr\u003e\nClone: A20079E\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Cluster of differentiation 276 (CD276), B7 Homolog 3 (B7-H3), 4Ig-B7-H3, B7RP-2\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866070208681,"sku":"616951","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-616951","provider":"Iright","version":"1.0","type":"link"}