{"product_id":"biolegend-622403","title":"Biolegend, 622403, Purified anti-p38 MAPK Antibody, 50microl","description":"\u003cp\u003ep38 mitogen-activated protein kinase (MAPK) is a member of the stress activated kinase family with a molecular weight of 38 kD. MAPK can be activated by a number of stimuli including heat, changes in osmolarity, irradiation, and cold. The MAPKs require dual phosphorylation (threonine and tyrosine) for enzymatic activation. The p38 MAP kinase is required for G2 cell cycle delay after UV irradiation, also participates in cytokine production (IL-6 and erythropoietin). The Poly6224 antibody recognizes human, mouse, and rat p38 MAPK and has been shown to be useful for Western blotting.\u003cbr\u003e\n50microl\u003cbr\u003e\nVerified Reactivity: Mouse, Rat, Human\u003cbr\u003e\nAntibody Type: Polyclonal\u003cbr\u003e\nHost Species: Rabbit\u003cbr\u003e\nImmunogen: peptide-KLH, C terminal\u003cbr\u003e\nFormulation: PBS containing 0.09% sodium azide and 0.2% gelatin.Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested IHC-P - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1:4000 dilution. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a dilution of 1:200 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This product may contain other non-IgG subtypes.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): da Silva, et al. 2011. Biochim Biophys Acta. 181:1863. PubMed Chen W, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 303:279. PubMed Maeda S, et al. 2014. J Biol Chem. 289:23786. PubMed\u003cbr\u003e\nProduct Citations: Amirova KM, et al. 2023. Int J Mol Sci. 24:. PubMed Jiang Q, et al. 2022. Stem Cells Int. 2022:5897194. PubMed\u003cbr\u003e\nRRID: AB_2876740 (BioLegend Cat. No. 622403)\u003cbr\u003e\nStructure: Belongs to serine\/threonine kinase family, MAP subfamily, 38 kD\u003cbr\u003e\nDistribution: Ubiquitously expressed, nuclear and cytoplasmic\u003cbr\u003e\nFunction: p38 MAPK plays a critical role in the initiation of G2 delay after ultraviolet radiation; gene knock-out studies have also revealed a critical role for p38 in cardiac remodeling. Downstream targets of p38 include the transcription factors ELK1 and ATF2, MEF2C and MAX.\u003cbr\u003e\nInteraction: Interacts with MAX, Cdc25B, Cdc25C. Binds to kinase interaction domain in the protein tyrosine phosphatase PTPRR; this interaction retains p38 MAPK in the cytoplasm.\u003cbr\u003e\nModification: Phosphorylation\u003cbr\u003e\nBiology Area: Cell Biology\u003cbr\u003e\nMolecular Family: Protein Kinases\/Phosphatase\u003cbr\u003e\nAntigen References: 1. Bulavin DV, et al. 2001. Nature 411:102. 2. Han J, et al. 1994. Science 265:808. 3. Lee JC, et al. 1994. Nature 372:739.\u003cbr\u003e\nRegulation: Activated by environmental stress, many pro-inflammatory cytokines and lipopolysaccharide. Dual phosphorylation by MAP2K3 and MAP2K6 is required for activation of p38 MAPK.\u003cbr\u003e\nGene ID: 80824\u003cbr\u003e\nUniProt: View information about p38 MAPK on UniProt.org\u003cbr\u003e\nClone: Poly6224\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Stress activated kinase p38, p38 MAP kinase, stress activated protein kinase 2A\u003cbr\u003e\nIsotype: Rabbit Polyclonal IgG\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866470764713,"sku":"622403","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-622403","provider":"Iright","version":"1.0","type":"link"}