{"product_id":"biolegend-623102","title":"Biolegend, 623102, Purified anti-IRF8 Antibody, 100μg","description":"\u003cp\u003eInterferon Regulatory Factor 8 (IRF8) is one of 9 members of the IRF transcription factor family that plays a critical role in the lineage commitment of myeloid cells. IRF8 has a DNA binding domain in its N-terminus region and an IRF association domain (IAD) in the C-terminus region. The IAD allows IRF8 to interact with other transcription factors and regulate gene expression. PU.1 is an IRF8 binding partner that forms a complex with IRF8 and mediates the differentiation of myeloid and lymphoid cells. The IRF8-PU.1 complex binds to a promoter-distal IRF8 binding site and induces the expression of KLF4, which drives the development of Ly6C+ monocytes. IRF8(-\/-) mice are immunodeficient and highly  susceptible to infections. They are also characterized by a Chronic Myelogenous Leukemia (CML)-like syndrome marked by an increased number of myeloid progenitor cells that can only commit toward the granulocyte but not toward the macrophage lineage.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial recombinant mouse IRF8 protein (amino acids 115-424)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality testedIP - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nRRID: AB_2814480 (BioLegend Cat. No. 623101) AB_2814481 (BioLegend Cat. No. 623102)\u003cbr\u003e\nStructure: Mouse IRF8 is a 424 amino acid protein with a predicted molecular weight of 48.2 kD.\u003cbr\u003e\nDistribution: Nucleus\/Myeolid and lymphoid tissue\u003cbr\u003e\nFunction: Transcription factor\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Transcription Factors\u003cbr\u003e\nMolecular Family: Innate Immune Signaling\u003cbr\u003e\nAntigen References: Kurotaki D, et al. 2018. Cell Reports. 22:2628. Tamura T, et al. 2005. Blood. 106:1938. Wang H, et al. 2009. Immunol. Res. 43(1-3):109.\u003cbr\u003e\nGene ID: 3394\u003cbr\u003e\nUniProt: View information about IRF8 on UniProt.org\u003cbr\u003e\nClone: W17160D\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interferon Regulatory Factor 8, Interferon Consensus Sequence Binding Protein 1, ICSBP, IRF-8, IMD32A, IMD32B\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866314657961,"sku":"623102","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-623102","provider":"Iright","version":"1.0","type":"link"}