{"product_id":"biolegend-623901","title":"Biolegend, 623901, Purified anti-LAT Antibody, 25μg","description":"\u003cp\u003eLAT (linker for activation of T-cells) is a 36 kD phospho-tyrosine adaptor protein containing SH2 domains and a CVRC motif. Two LAT isoforms have been reported with approximate molecular weights of 36 kD and 38 kD. LAT is localized to lipid rafts, and is also a potential type III transmembrane protein, thought to be expressed in the juxtanuclear intracellular compartment. LAT links TCR engagement to Ras-MAPK activation, Ca 2+ flux, IL-2 expression and is required for T-cell development. LAT is phosphorylated when activated by TCR cross-linking, where it recruits signaling molecules. LAT activation can be prevented by polyunsaturated fatty acids. LAT is modified by ZAP-70 and Syk phosphorylation and can also be palmitoylated. LAT has been reported to be associated with Grb2, Gads, Grap, PLC-γ1, p85 subunit PI3K, ZAP-70, Syk, Itk, Vav, SLP-76, and cbl.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Partial recombinant protein (cytoplasmic domain of human LAT)\u003cbr\u003e\nFormulation: This LAT antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg\/ml.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, store between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICFC - Verified IP - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: The LAT1111 monoclonal antibody recognizes the cytoplasmic domain of human LAT and has been shown to be useful for Western blotting and immunoprecipitation. The LAT1111 antibody does not cross-react with mouse LAT.\u003cbr\u003e\nProduct Citations: Ghosh S, et al. 2020. Cell Reports. 30(10):3434-3447. PubMed\u003cbr\u003e\nRRID: AB_2133119 (BioLegend Cat. No. 623901) AB_2133118 (BioLegend Cat. No. 623902)\u003cbr\u003e\nStructure: SH2 domains, CVRC motif; two isoforms 36 kD, 38 kD\u003cbr\u003e\nDistribution: Lipid rafts, potential type III transmembrane protein, juxtanuclear intracellular compartment\u003cbr\u003e\nFunction: Links TCR engagement to Ras-MAPK activation, Ca2+ flux, IL-2 expression; required for T-cell development\u003cbr\u003e\nInteraction: Grb2, Gads, Grap, PLC-γ1, p85 subunit PI3K, ZAP-70, Syk, Itk, Vav, SLP-76, cbl\u003cbr\u003e\nModification: Phosphorylation by ZAP-70, Syk, Palmitoylated\u003cbr\u003e\nCell Type: Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Signal Transduction\u003cbr\u003e\nMolecular Family: Phospho-Proteins\u003cbr\u003e\nAntigen References: 1. Zhang W, et al. 1998. Cell. 92:83. 2. Zeyda M, et al. 2002. J. Biol. Chem. 277:28418. 3. Perez-Villar J, et al. 2002 Biochemistry. 41:10732. 4. Hartgroves L, et al. 2003 J. Biol. Chem. 278:20389.\u003cbr\u003e\nRegulation: Phosphorylated when activated by TCR cross-linking, recruits signaling molecules, activation prevented by polyunsaturated fatty acids\u003cbr\u003e\nGene ID: 27040\u003cbr\u003e\nUniProt: View information about LAT on UniProt.org\u003cbr\u003e\nClone: LAT1111\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Linker for activation of T-cells, 36 kD phospho-tyrosine adaptor protein\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866459197609,"sku":"623901","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-623901","provider":"Iright","version":"1.0","type":"link"}