{"product_id":"biolegend-624852","title":"Biolegend, 624852, Purified anti-SMAD2 Antibody, 100μg","description":"\u003cp\u003eSMAD2 is a potent regulator of different cellular processes such as apoptosis, differentiation, and cell proliferation. SMAD2 is associated with SARA protein and the TGF-β receptor. SMAD2 gets phosphorylated by the TGF-β receptor in response to TGF-β signaling, which induces its dissociation from SARA and association with SMAD4 followed by translocation into the nucleus. In the nucleus, the SMAD2\/SMAD4 protein complex participates in gene regulation by forming a transcription repressor complex in association with other cofactors.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant human SMAD2 protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg\/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.016 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This clone cross-reacts with mouse. For intracellular flow cytometric staining with this clone, we recommended using Fixation Buffer (Cat. No. 420801), True-Phos™ Perm Buffer (Cat. No. 425401), Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002) or Cyto-Fast™ Fix\/Perm Buffer Set (Cat. No. 426803). True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) is not applicable. We do not recommend this product for use in immunocytochemistry (ICC).\u003cbr\u003e\nRRID: AB_3097247 (BioLegend Cat. No. 624851) AB_3097247 (BioLegend Cat. No. 624852)\u003cbr\u003e\nStructure: SMAD2 is a 467 amino acid protein with a predicted molecular weight of 52 kD\u003cbr\u003e\nDistribution: Skeletal muscle, endothelial cells, heart and placenta; non-phosphorylated SMAD2 is present in the cellular cytoplasm close to the TGF-β receptor; phosphorylated SMAD2 is present in the cellular nucleus and binds to target promoter sequences.\u003cbr\u003e\nFunction: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Cycle\/DNA Replication, Signal Transduction, Transcription Factors\u003cbr\u003e\nInteraction: TAZ\/WWRT1, FOXH1, SNW1, CREB-binding protein (CBP), EP300, SNON, ALK4\/ACVR1B, SKOR1, SKOR2, PRDM16, LEMD3, RBPMS, WWP1, PDPK1, DAB2, USP15, PPP5C, LDLRAD4, PMEPA1, ZFHX3, ZNF451, SMURF2, PPM1A, TGF-β, TGFBR1, TGIF, SMAD3, TRIM33, ZNF580, NEDD4, HGS, AIP1, WWP1, PML, ZNF8, RNF111, YAP1\u003cbr\u003e\nLigand\/Receptor: SMAD2 is part of the TGF-β signaling pathway but it is not a ligand\/receptor protein\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Cycle\/DNA Replication, Signal Transduction, Transcription Factors\u003cbr\u003e\nMolecular Family: Phospho-Proteins\u003cbr\u003e\nAntigen References: Nakao A, et al. 1997. EMBO J. 16:5353–62. Attisano L. and Wrana JL, et al. 2002. Science. 296:1646-47. Moustakas A. et al. 2001. J Cell Sci. 114: 4359-69.\u003cbr\u003e\nGene ID: 4087\u003cbr\u003e\nUniProt: View information about SMAD2 on UniProt.org\u003cbr\u003e\nClone: W21150C\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: MAD homolog 2, JV18-1, MAD-2, MADH2, MADR2, Mad-related protein 2, Mothers against DPP homolog 2\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866430918825,"sku":"624852","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-624852","provider":"Iright","version":"1.0","type":"link"}