{"product_id":"biolegend-625852","title":"Biolegend, 625852, Purified anti-ATG7 Antibody, 100μg","description":"\u003cp\u003eATG7 is an E1-like ligase that plays a central role in autophagosome biogenesis by conjugating ATG5 to ATG12 which degrades and recycles damaged organelles, protein aggregates, and invading pathogens. ATG7 interacts with other ATG proteins, such as ATG3, ATG10, and ATG16L1, to form a complex that is required for autophagy, also regulates immunity, cell death, and protein secretion, and independently regulates the cell cycle and apoptosis. It also participates in lipidation of the ATG8 protein, which is essential for autophagosome formation. Additionally, ATG7 has been shown to interact with other proteins outside of the autophagy pathway, such as p53, to regulate apoptosis. Disrupting ATG7-dependent autophagy has been shown to cause electromotility disturbances, outer hair cell loss, and deafness in mice. ATG7 dysfunction has been associated with various human diseases, including cancer, neurodegeneration, and infection. ATG7 dysfunction and disease was found in patients with biallelic ATG7 variants and childhood-onset neuropathology. Furthermore, ATG7 has been shown to induce basal autophagy and rescue autophagic deficiency in CryAB R120G cardiomyocytes. This product was carefully developed for performance and specificity using the following products from Horizon Discovery, a Revvity company:  HAP1 ATG KO cells (Horizon Cat. No. HZGHC000302c022)\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant fragment of human ATG7\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC, IHC-P, KO\/KD-WB - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg\/mL. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg\/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg\/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nAdditional Product Notes: For immunohistochemistry (IHC-P), Citrate Buffer, 10X (Cat. No, 420902) is recommended. For immunocytochemistry (ICC), either Fixation Buffer (Cat. No. 420801) or paraformaldehyde-based fixation is recommended. Clone A17129A detects homodimer of ATG7 in western blotting (WB). The homodimer band is demolished by increasing DTT concentration in the lysis buffer.\u003cbr\u003e\nRRID: AB_3083415 (BioLegend Cat. No. 625851) AB_3083415 (BioLegend Cat. No. 625852)\u003cbr\u003e\nStructure: Homodimer\u003cbr\u003e\nDistribution: Cytosol and preautophagosomal structure\u003cbr\u003e\nFunction: Conjugate ATG12 to ATG5, lipidation of ATG8\u003cbr\u003e\nInteraction: Interacts with ATG3, ATG8, ATG12, EP300 and FOXO1\u003cbr\u003e\nCell Type: Mature Neurons, Neurons\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Cell Death, Cell Proliferation and Viability, Neurodegeneration, Protein Misfolding and Aggregation, Protein Trafficking and Clearance, Ubiquitin\/Protein Degradation\u003cbr\u003e\nMolecular Family: Autophagosome Markers, Ligases\u003cbr\u003e\nAntigen References: Komatsu M, et al. 2005. J Cell Biol. 169:425-34. Komatsu M, et al. 2007. Proc Natl Acad Sci USA. 104:14489-94. Lee I H, et al. 2012. Science. 336: 225–228. Collier JJ, et al. 2021. EMBO Mol Med. 13:e14824.\u003cbr\u003e\nGene ID: 10533\u003cbr\u003e\nUniProt: View information about ATG7 on UniProt.org\u003cbr\u003e\nClone: A17129A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: APG7, APG7-like, APG7L, Ubiquitin-activating enzyme E1-like protein, Ubiquitin-Like Modifier-Activating Enzyme ATG7, Autophagy-related protein 7\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866051334313,"sku":"625852","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-625852","provider":"Iright","version":"1.0","type":"link"}