{"product_id":"biolegend-626804","title":"Biolegend, 626804, Direct-Blot™ HRP anti-c-Myc Antibody, 25microl","description":"\u003cp\u003eThe 9E10 monoclonal antibody recognizes the amino acid sequence EQKLISEEDL, which is a specific portion of the human c-myc gene product. This antibody is highly specific and is unlikely to alter the activity of the cloned sequence.\u003cbr\u003e\n25microl\u003cbr\u003e\nVerified Reactivity: Epitope tag\u003cbr\u003e\nReported Reactivity: Species independent\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Amino acids 408-439, C-terminal region of human c-myc\u003cbr\u003e\nFormulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest. 25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration\/dilution.\u003cbr\u003e\nApplication Notes: *Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Rao H, et al. 2001. Nature. 410:955. Peters CS, et al. 2001. J Biol Chem. 276:13718. Munro S, Pelham HR. 1987. Cell. 48:899. Evan GI, et al. 1985. Mol Cell Biol. 5:3610.\u003cbr\u003e\nProduct Citations: Calvo IA, et al. 2021. iScience. 24:103338. PubMed\u003cbr\u003e\nRRID: AB_2783361 (BioLegend Cat. No. 626804) AB_2572009 (BioLegend Cat. No. 626803)\u003cbr\u003e\nBiology Area: Cell Biology, Protein Purification, Protein Synthesis\u003cbr\u003e\nAntigen References: Adams JM, et al. 1983. Proc. Natl. Acad. Sci. USA 80:1982. Atchley WR, et al. 1995. Proc. Natl. Acad. Sci. USA 92:10217. Battey J, et al. 1983. Cell 34:779. Beimling P, et al. 1985. Biochemistry 24:6349.\u003cbr\u003e\nGene ID: 4609\u003cbr\u003e\nUniProt: View information about c-Myc on UniProt.org\u003cbr\u003e\nClone: 9E10\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: myc tag, c-Myc tag, cMyc tag, Myc epitope tag\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862506950825,"sku":"626804","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-626804","provider":"Iright","version":"1.0","type":"link"}