{"product_id":"biolegend-627101","title":"Biolegend, 627101, Purified anti-NTAL Antibody, 25μg","description":"\u003cp\u003eNon-T cell activation Linker (NTAL) is a transmembrane adaptor protein with an apparent molecular weight of 30 kD expressed on B cells, natural killer (NK) cells, and myeloid cells. The NTAL protein is found in rafts, which are membrane microdomains that participate in B cell activation. NTAL interacts with a variety of proteins including Grb2, PLC gamma 2, and PLC gamma 1. This gene is one of the contiguous genes at 7q11.23 commonly deleted in Williams syndrome.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant cytoplasmic domain (91-243 a.a.) of human NTAL.\u003cbr\u003e\nFormulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, store undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested IHC-P - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. For IHC, use a 15 µl\/ml dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.\u003cbr\u003e\nProduct Citations: Brühl H, et al. 2014. J Leukoc Biol. 65:96. PubMed\u003cbr\u003e\nRRID: AB_2133251 (BioLegend Cat. No. 627102)\u003cbr\u003e\nStructure: 30 kD double palmitoylated protein gene that consists of 14 exons, and its alternative splicing generates three transcript variants.\u003cbr\u003e\nDistribution: B, natural killer cells, monocytes, mast cells, and activated T cells.\u003cbr\u003e\nFunction: Involved in FCER1 mediated signaling in mast cells and as a negative regulator of early B cell signaling. May also be involved in CFCGR1 mediated signaling in myeloid cells. Couples activation of these receptors with distal intracellular events through the recruitment of Grb2.\u003cbr\u003e\nLigand\/Receptor: Membrane microdomains (rafts) of B cells, natural killer (NK) cells, and myeloid cells.\u003cbr\u003e\nCell Type: B cells, Mast cells, Monocytes, NK cells, T cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Signal Transduction\u003cbr\u003e\nAntigen References: 1. Yamasaki S, et al. 2007. Mol. Cell Biol. 27(12):4406-15. 2. Brdicka T, et al. 2002. J. Exp. Med. 196(12):1617-26. 3. Tkaczyk C, et al. 2004. Blood 104(1):207-14. 4. Volna P, et al. 2004. 200(8):1001-13. 5. Lebduska P, et al. 2007. 328(1-2):139-51.\u003cbr\u003e\nGene ID: 7462\u003cbr\u003e\nUniProt: View information about NTAL on UniProt.org\u003cbr\u003e\nClone: NAP-07\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: LAT2 (linker for activation of T cells family), member 2, LAB\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866465030313,"sku":"627101","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-627101","provider":"Iright","version":"1.0","type":"link"}