{"product_id":"biolegend-627701","title":"Biolegend, 627701, Purified anti-PLK-1 Antibody, 25μg","description":"\u003cp\u003ePLK-1 (polo-like kinase 1) is a member of te serine\/threonine protein kinase family, cdc5\/polo subfamily. Highly homologous to polo-like kinase (Drosophila), PLK-1 contains two polo box domains with a predicted molecular weight of 68 kD. This nuclear protein is highly expressed in placenta and colon and has been shown to regulate cdc2\/cyclin B through phosphorylation and activation of cdc25c phosphatase. PLK-1 may also be required for cell division; depletion of PLK-1 results in apoptosis. PLK-1 is upregulated by growth stimulating agents and is regulated by cell cycle position (highest in G2\/M phase, declining to nearly undetectable levels after mitosis and throughout G1). PLK-1 is modified by phosphorylation (Thr210 is the major phosphorylation site in activated PLK-1 from mitotic cells) and has been shown to interact with nuclear distribution gene C. The 3F8 monoclonal antibody recognizes human and mouse PLK-1 and has been shown to be useful for Western blotting.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Amino Acid: 300-603 of human PLK-1\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested KO\/KD-WB - Verified IP, ICC - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1.0 - 5.0 µg (1:100-1:500) antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nProduct Citations: Chilamakuri R, et al. 2022. Pharmaceuticals (Basel). 15:. PubMed Zhu K, et al. 2022. Cell Death Differ. 2070:29. PubMed Rajamanickam S, et al. 2016. Clin Cancer Res. 22: 3524 - 3536. PubMed Auler M, et al. 2020. J Invest Dermatol. . PubMed\u003cbr\u003e\nRRID: AB_439756 (BioLegend Cat. No. 627701) AB_439757 (BioLegend Cat. No. 627702)\u003cbr\u003e\nStructure: Serine\/Threonine family of protein kinases, cdc5\/polo subfamily. Highly homologous to polo-like kinase (Drosophila). Contains two polo box domains. Predicted molecular weight 68 kD\u003cbr\u003e\nDistribution: Nuclear protein, highly expressed in placenta and colon\u003cbr\u003e\nFunction: Regulates cdc2\/cyclin B through phosphorylation and activation of cdc25c phosphatase. May be required for cell division. Depletion of PLK-1 results in apoptosis\u003cbr\u003e\nInteraction: Interacts with nuclear distribution gene C\u003cbr\u003e\nModification: Phosphorylation\u003cbr\u003e\nBiology Area: Cell Biology, Cell Cycle\/DNA Replication, Signal Transduction\u003cbr\u003e\nMolecular Family: Protein Kinases\/Phosphatase\u003cbr\u003e\nAntigen References: 1. Hamanaka R, et al. 1994. Cell Growth Differ. 5:249. 2. Lake RJ, et al. 1993. Mol. Cell. Biol. 13:7793. 3. Holtrich U, et al. 1994. P. Natl. Acad. Sci. USA 91:1736.\u003cbr\u003e\nRegulation: Upregulated by growth stimulating agents. Regulated by cell cycle position (highest in G2\/M phase and declines to nearly undetectable levels after mitosis and throughout G1)\u003cbr\u003e\nGene ID: 5347\u003cbr\u003e\nUniProt: View information about PLK-1 on UniProt.org\u003cbr\u003e\nClone: 3F8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Serine\/Threonine protein kinase PLK, Polo-like kinase (PLK), Serine-threonine protein kinase 13\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862395932841,"sku":"627701","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-627701","provider":"Iright","version":"1.0","type":"link"}