{"product_id":"biolegend-627902","title":"Biolegend, 627902, Purified anti-Tubulin-α Antibody, 100μg","description":"\u003cp\u003eTubulin, one of the major constituents of microtubules, is a dimeric protein consisting of an alpha and beta chain. Tubulin is a highly conserved protein that is ubiquitously expressed. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation. Tubulin interacts with a variety of proteins including RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, and microtubule associated protein 1A. The 10D8 antibody recognizes α-tubulin in all species and is useful for Western blotting, and immunofluorescence staining. The 10D8 antibody can also be used to monitor protein loading in Western blotting.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat, All Species\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant full length human α-tubulin\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested IHC-P - Verified ICC - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2 - 10 µg\/mL is suggested. For immunofluorescent staining applications, a concentration range of 1 - 4 µg\/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Chou HYE, et al. 2006. J. Biol. Chem. 281:15201.\u003cbr\u003e\nProduct Citations: Bian S, et al. 2021. Exp Ther Med. 22:667. PubMed Gärtner K, et al. 2018. Cancer Med. 11:39. PubMed Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed Kogut S, et al. 2022. Epigenomes. 6:. PubMed Rodems TS, et al. 2022. Commun Biol. 5:897. PubMed Yadav B, et al. 2021. PLoS Pathog. 17:e1009891. PubMed\u003cbr\u003e\nRRID: AB_439760 (BioLegend Cat. No. 627901) AB_439761 (BioLegend Cat. No. 627902)\u003cbr\u003e\nStructure: Tubulin family, tubulin is a dimeric protein consisting of an alpha and beta chain. Alpha tubulin has an approximate molecular weight of 50-55 kD.\u003cbr\u003e\nDistribution: Ubiquitous\u003cbr\u003e\nFunction: Tubulin is one of the major constituents of microtubules. Binds GTP, alpha chain contains a non-exchangeable GTP binding site\u003cbr\u003e\nInteraction: RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, Microtubule associated protein 1A\u003cbr\u003e\nModification: Phosphorylation, acetylation\u003cbr\u003e\nBiology Area: Cell Biology, Cell Motility\/Cytoskeleton\/Structure, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: Microtubules\u003cbr\u003e\nAntigen References: 1. Cowan NJ, et al. 1983. Mol. Cell. Biol. 3:1738. 2. Miller FD, et al. 1987. J. Cell Biol. 105:3065. 3. Wilde CD, et al. 1982. Proc. Natl. Acad. Sci. USA 79:96.\u003cbr\u003e\nGene ID: 84790\u003cbr\u003e\nUniProt: View information about Tubulin-alpha on UniProt.org\u003cbr\u003e\nClone: 10D8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: α-tubulin, Tubulin, alpha-ubiquitous chain\u003cbr\u003e\nIsotype: Mouse IgM, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860779978921,"sku":"627902","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-627902","provider":"Iright","version":"1.0","type":"link"}