{"product_id":"biolegend-628602","title":"Biolegend, 628602, Purified anti-Cytokeratin (pan reactive) Antibody, 100μg","description":"\u003cp\u003eCytokeratins are intermediate filament proteins that are widely expressed in many tissues. These proteins are vital components of the cytoskeleton and interact with a number of other proteins. Cytokeratins are usually found in the cytoplasm,although some cytokeratins can be found in the nucleus and the nucleolus.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Keratin enriched fraction from human epidermoid carcinoma.\u003cbr\u003e\nFormulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg\/ml.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC - Verified IP, IHC-P - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration range of 1.0 - 4.0 μg\/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: The C-11 monoclonal antibody reacts with a conserved epitope of human cytokeratin 4, 5, 6, 8, 10, 13, and 18. This antibody has been shown to be useful for Western blotting and has also been reported to be useful for immunoprecipitation, immunohistochemistry (paraffin sections), immunocytochemistry, and spatial biology (IBEX)7,8.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kovarik J. 1988. Int. J. Cancer Suppl. 3:50. Bartek J, et al. 1991. J. Pathol. 164:215. Chernyavsky A I, 2007. J. Biol. Chem. doi:10.1074\/jbc.M611365200. PubMed Comito G, et al. 2012. Cancer Lett. 324:31. PubMed Vojt-òsek B, et al. 1989. Folia Biol (Praha). 35:373. (reactivity with rat, dog, sheep, pig, cow) Dekaney CM, et al. 2005. Gastroenterology 129:1567. (ICC) Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Yamashita S, et al. 2018. Oncol Lett. 15:400. PubMed Greier MDC, et al. 2023. Front Oncol. 13:1145817. PubMed Fang WB, et al. 2023. Front Oncol. 13:1130911. PubMed Nwosu ZC, et al. 2023. Nature. 618:151. PubMed Diray-Arce J, et al. 2023. Cell Rep Med. 4:101079. PubMed Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed Florian S, et al. 2019. Proc Natl Acad Sci U S A. 116:11444. PubMed Kesavan R, et al. 2021. Int J Mol Sci. 22:. PubMed Yao Y, et al. 2017. Cytometry B Clin Cytom. 92:12:00. PubMed Comito G, et al. 2012. Cancer Lett. 324:31. PubMed Baines KJ, et al. 2020. J Immunol. 204:694. PubMed Chernyavsky A, et al. 2007. J Biol Chem . 2.042361111. PubMed Bade RM, et al. 2021. Molecular Oncology. . PubMed Mayer J, et al. 2011. Cancer Genet. 204:589. PubMed Ran LJ, et al. 2018. Mol Med Rep. 18:723. PubMed Mukhtar E, et al. 2020. Cancer Drug Resist. 0.566666667. PubMed\u003cbr\u003e\nRRID: AB_439774 (BioLegend Cat. No. 628601) AB_439775 (BioLegend Cat. No. 628602)\u003cbr\u003e\nStructure: Intermediate filament protein, contains three coiled-coil domains. Cytokeratins exist as heterotetramers composed of two type I and two type II keratin subunits\u003cbr\u003e\nDistribution: Cytokeratins are widely expressed proteins usually found in the cytoplasm although some cytokeratins can also be found in the nucleus and the nucleolus\u003cbr\u003e\nFunction: Intermediate filament protein involved with the cytoskeleton.\u003cbr\u003e\nInteraction: Most cytokeratins interact with a number of other proteins such as 14-3-3, EGF receptor, and many others\u003cbr\u003e\nBiology Area: Cell Biology, Cell Motility\/Cytoskeleton\/Structure, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: Intermediate Filaments\u003cbr\u003e\nGene ID: 3875\u003cbr\u003e\nUniProt: View information about Cytokeratin pan reactive on UniProt.org\u003cbr\u003e\nClone: C-11\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Cytokeratin, Keratin\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866452218025,"sku":"628602","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-628602","provider":"Iright","version":"1.0","type":"link"}