{"product_id":"biolegend-628901","title":"Biolegend, 628901, Purified anti-PLK-1 Phospho (Thr210) Antibody, 25μg","description":"\u003cp\u003ePLK-1 (polo-like kinase 1) is a member of te serine\/threonine protein kinase family, cdc5\/polo subfamily. Highly homologous to polo-like kinase (Drosophila), PLK-1 contains two polo box domains with a predicted molecular weight of 68 kD. This nuclear protein is highly expressed in placenta and colon and has been shown to regulate cdc2\/cyclin B through phosphorylation and activation of cdc25c phosphatase. PLK-1 may also be required for cell division; depletion of PLK-1 results in apoptosis. PLK-1 is upregulated by growth stimulating agents and is regulated by cell cycle position (highest in G2\/M phase, declining to nearly undetectable levels after mitosis and throughout G1). PLK-1 is modified by phosphorylation (Thr210 is the major phosphorylation site in activated PLK-1 from mitotic cells) and has been shown to interact with nuclear distribution gene C. The 2A3 antibody recognizes human phosphorylated PLK-1 (Thr210) and has been shown to be useful for Western blotting. To increase specificity, it is recommended that the 2A3 antibody be used for Western blotting after immunoprecipitation with the pan-specific PLK-1 3F8 antibody.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Modified peptide\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 mL antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Molli PR, et al. 2010. J Cell Biol. 190:101. PubMed Yang S, et al. 2013. Cancer Res. 73:6722. PubMed\u003cbr\u003e\nProduct Citations: Pop OT, et al. 2023. Cell Mol Gastroenterol Hepatol. 17:16. PubMed Bucko PJ, et al. 2019. eLife. 0.333333333333333. PubMed Bucko PJ, et al. 2020. J Biol Chem. 295:13784. PubMed Yang S, et al. 2013. Cancer Res. 73:6722. PubMed Molli P, et al. 2010. J Cell Biol. 190:101. PubMed Tankiewicz‐Kwedlo A et al. 2017. British journal of pharmacology. 175(5):743-762 . PubMed\u003cbr\u003e\nRRID: AB_439786 (BioLegend Cat. No. 628901) AB_439787 (BioLegend Cat. No. 628902)\u003cbr\u003e\nStructure: Serine\/Threonine family of protein kinases, cdc5\/polo subfamily. Highly homologous to polo-like kinase (Drosophila). Contains two polo box domains. Predicted molecular weight 68 kD\u003cbr\u003e\nDistribution: Nuclear protein, highly expressed in placenta and colon\u003cbr\u003e\nFunction: Regulates cdc2\/cyclin B through phosphorylation and activation of cdc25c phosphatase. May be required for cell division. Depletion of PLK-1 results in apoptosis\u003cbr\u003e\nInteraction: Interacts with nuclear distribution gene C\u003cbr\u003e\nModification: Phosphorylation (Thr210 is major phosphorylation site in activated PLK-1 from mitotic cells)\u003cbr\u003e\nBiology Area: Cell Biology, Cell Cycle\/DNA Replication, Signal Transduction\u003cbr\u003e\nMolecular Family: Phospho-Proteins, Protein Kinases\/Phosphatase\u003cbr\u003e\nAntigen References: 1. Hamanaka R, et al. 1994. Cell Growth Differ. 5:249. 2. Lake RJ, et al. 1993. Mol. Cell. Biol. 13:7793. 3. Holtrich U, et al. 1994. P. Natl. Acad. Sci. USA 91:1736.\u003cbr\u003e\nRegulation: Upregulated by growth stimulating agents. Regulated by cell cycle position (highest in G2\/M phase and declines to nearly undetectable levels after mitosis and throughout G1)\u003cbr\u003e\nGene ID: 5347\u003cbr\u003e\nUniProt: View information about PLK-1 Phospho Thr210 on UniProt.org\u003cbr\u003e\nClone: 2A3\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: HCDM listed\u003cbr\u003e\nOther Names: Serine\/Threonine protein kinase PLK, Polo-like kinase (PLK), Serine-threonine protein kinase 13\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866551996585,"sku":"628901","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-628901","provider":"Iright","version":"1.0","type":"link"}